不溶性跨膜肽的制备：血型糖蛋白A、朊病毒（110 - 137）和成纤维细胞生长因子受体（368 - 397）
Preparation of insoluble transmembrane peptides: glycophorin-A, prion (110-137), and FGFR (368-397).
作者信息
Glover K J, Martini P M, Vold R R, Komives E A
机构信息
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0601, USA.
出版信息
Anal Biochem. 1999 Aug 1;272(2):270-4. doi: 10.1006/abio.1999.4182.
A general procedure for the reliable preparation of large quantities of insoluble transmembrane peptides has been developed. Optimal couplings were obtained during synthesis by using high-temperature couplings in conjunction with O-(7-azabenzotriazol-1-yl)-1,1,3, 3-tetramethyluronium hexafluorophosphate (HATU) activation. Improved purification schemes were developed that use reverse- phase HPLC on a C1 column and elution with a 2:1 mixture of 1-propanol:1-butanol. Using these methods three very different transmembrane peptides all longer than 25 amino acids have been prepared: glycophorin-A, prion (110-137), and fibroblast growth factor receptor (368-397).
已开发出一种可靠地大量制备不溶性跨膜肽的通用方法。在合成过程中，通过使用高温偶联结合O-(7-氮杂苯并三唑-1-基)-1,1,3,3-四甲基脲六氟磷酸盐（HATU）活化，获得了最佳偶联效果。开发了改进的纯化方案，该方案使用C1柱上的反相高效液相色谱，并以1-丙醇:1-丁醇的2:1混合物进行洗脱。使用这些方法已制备出三种长度均超过25个氨基酸的截然不同的跨膜肽：血型糖蛋白-A、朊病毒（110-137）和成纤维细胞生长因子受体（368-397）。
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