用于优化疏水相互作用的实验模型：胰高血糖素的色谱纯化

Experimental model for optimization of hydrophobic interaction: chromatographic purification of glucagon.

作者信息

Dimov N, Angelova M

机构信息

Chemical Pharmaceutical Research Institute, NIHFI, Sofia, Bulgaria.

出版信息

Biomed Chromatogr. 1999 Jun;13(4):304-8. doi: 10.1002/(SICI)1099-0801(199906)13:4<304::AID-BMC870>3.0.CO;2-1.

DOI:10.1002/(SICI)1099-0801(199906)13:4<304::AID-BMC870>3.0.CO;2-1

PMID:10416065

Abstract

The chromatographic behavior of the components of glucagon-containing material from bovine pancreas by hydrophobic interaction on Amberlite XAD-7 was investigated. The effect of initial ionic strength and pH of the mobile phase on selectivity was quantitatively determined. The data, obtained from 56 experiments, were used for modeling and the corresponding management of the purification process. The purified glucagon is of very high quality: total impurities are not more than 1.7% as determined by analytical HPLC. The biological activity is 1.08 IU/mg, calculated for dry substance.

摘要

研究了牛胰脏中含胰高血糖素物质的成分在Amberlite XAD - 7上通过疏水相互作用的色谱行为。定量测定了流动相的初始离子强度和pH对选择性的影响。从56次实验获得的数据用于建模和纯化过程的相应管理。纯化的胰高血糖素质量非常高：通过分析型高效液相色谱法测定，总杂质不超过1.7%。以干物质计算，生物活性为1.08 IU/mg。