Yamaguchi T, Hiratsuka M, Asai K, Kai K, Kumagai K
Department of Animal Science, Faculty of Agriculture, Tohoku University, Sendai, Japan.
J Dairy Sci. 1999 Jul;82(7):1459-64. doi: 10.3168/jds.S0022-0302(99)75373-7.
The existence and distribution of T lymphocyte subpopulations in the mammary parenchymal tissue of cows were immunohistochemically detailed during early lactation. CD2+, CD4+, and CD8+ T lymphocytes were localized primarily in the mammary parenchymal tissue. CD8+ T lymphocytes were predominant over CD4+ T lymphocytes and occurred in close contact with the alveolar epithelium and between epithelial cells in the central area of the upper mammary gland. CD4+ T lymphocytes were present in equal numbers in the epithelial and connective tissue area. Occasionally, both CD4+ and CD8+ T lymphocytes formed cell clusters in the interalveolar connective tissue. The ratio of CD4+ T lymphocytes to CD8+ T lymphocytes was less than 1.0 and was lower in the epithelial area than in the connective tissue. The distribution of CD4+ and CD8+ T lymphocytes was similar in the parenchymal tissue of the gland cistern. The observation that there is a preferential presence of CD8+ T lymphocytes in the epithelial area of the bovine mammary gland during early lactation might indicate that these cells participate in the maintenance of the integrity of the epithelium.
在奶牛泌乳早期，通过免疫组织化学方法详细研究了乳腺实质组织中T淋巴细胞亚群的存在和分布情况。CD2⁺、CD4⁺和CD8⁺ T淋巴细胞主要定位于乳腺实质组织中。CD8⁺ T淋巴细胞比CD4⁺ T淋巴细胞更为常见，且与乳腺腺泡上皮紧密接触，并出现在乳腺上部中央区域上皮细胞之间。CD4⁺ T淋巴细胞在上皮组织和结缔组织区域数量相当。偶尔，CD4⁺和CD8⁺ T淋巴细胞在腺泡间结缔组织中形成细胞簇。CD4⁺ T淋巴细胞与CD8⁺ T淋巴细胞的比例小于1.0，且上皮区域低于结缔组织区域。CD4⁺和CD8⁺ T淋巴细胞在腺池实质组织中的分布相似。奶牛泌乳早期乳腺上皮区域优先存在CD8⁺ T淋巴细胞这一观察结果可能表明这些细胞参与维持上皮的完整性。
