Loker E S, Coustau C, Ataev G L, Jourdane J
Department of Biology, University of New Mexico, Albuquerque 87131, USA.
Parasite. 1999 Jun;6(2):169-74. doi: 10.1051/parasite/1999062169.
Rediae of Echinostoma caproni (Egyptian strain) were dissected from Biomphalaria glabrata snails at intervals from 13-34 days post-exposure and co-cultured for up to 51 days with cells of the B. glabrata embryonic (Bge) cell line. Rediae readily ingested Bge cells and survived longer when co-cultured with cells than in cell-free cultures. Rediae released mostly motile cercariae throughout the observation period when in Bge medium and cells. Rediae cultured in 199 medium with Bge cells also produced progeny throughout most of the observation period. In the latter medium, progeny were much more likely to include rediae as well as cercariae. Some cercariae produced in vitro encysted as metacercariae. Rediae consumed cercariae released into culture but were not observed to attack one another or rediae of a different echinostome species.
在暴露后13至34天的不同时间间隔，从光滑双脐螺中解剖出卡氏棘口吸虫（埃及株）雷蚴，并与光滑双脐螺胚胎（Bge）细胞系的细胞共培养长达51天。雷蚴很容易摄取Bge细胞，与细胞共培养时比在无细胞培养中存活时间更长。在Bge培养基和细胞中，雷蚴在整个观察期内大多释放出活动的尾蚴。在含有Bge细胞的199培养基中培养的雷蚴在大部分观察期内也产生了后代。在后一种培养基中，后代更有可能包括雷蚴和尾蚴。一些在体外产生的尾蚴会形成囊蚴。雷蚴会消耗释放到培养物中的尾蚴，但未观察到它们相互攻击或攻击不同棘口吸虫物种的雷蚴。
