Crystallization and preliminary crystallographic study of HIP/PAP, a human C-lectin overexpressed in primary liver cancers.

作者信息

Abergel C, Chenivesse S, Stinnakre M G, Guasco S, Bréchot C, Claverie J M, Devinoy E, Christa L

机构信息

Information Génétique et Structurale, CNRS UMR 1889, Institut de Biologie Structurale et Microbiologie, 31 Chemin Joseph Aiguier, 13402 Marseille CEDEX 20, France.

出版信息

Acta Crystallogr D Biol Crystallogr. 1999 Aug;55(Pt 8):1487-9. doi: 10.1107/s0907444999007969.

Human HIP/PAP is an adhesion protein expressed in normal pancreatic and Paneth cells and overexpressed in hepatocellular carcinoma. HIP/PAP was crystallized using the Hampton Research Crystal Screen and SAmBA software to define the optimal crystallization protocol. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 30.73, b = 49.35, c = 92.15 A and one molecule in the asymmetric unit. Flash-frozen crystals diffract to 1. 78 A resolution using synchrotron radiation. A molecular-replacement solution was obtained using the human Reg/lithostathine structure and the AMoRe software.