Huyton T, Roper D I
Structural Biology Laboratory, Department of Chemistry, The University of York, Heslington, York YO10 5DD, England.
Acta Crystallogr D Biol Crystallogr. 1999 Aug;55(Pt 8):1481-3. doi: 10.1107/s0907444999007155.
A recombinant form of Enterococcus facieum BM4147 D-alanine-D-lactate ligase (VanA) has been prepared and crystallized. VanA was found to crystallize only in the presence of a phosphinate inhibitor analogue of D-alanine-D-alanine. The crystals grow in 40-45% ammonium sulfate, 0.1 M 3-(N-morpholino)-propanesulfonic acid pH 6.0 and reach dimensions of 0.4 x 0.2 x 0.1 mm. The crystals diffract to at least 2.5 A and are in the centred orthorhombic space group C222(1), with unit-cell dimensions a = 123.2, b = 225.4, c = 72.4 A.
已制备并结晶出屎肠球菌BM4147 D-丙氨酸-D-乳酸连接酶（VanA）的重组形式。发现VanA仅在D-丙氨酸-D-丙氨酸的次膦酸酯抑制剂类似物存在下结晶。晶体在40 - 45%硫酸铵、0.1 M 3-（N-吗啉代）-丙烷磺酸pH 6.0中生长，尺寸达到0.4×0.2×0.1毫米。晶体衍射至至少2.5 Å，属于体心正交空间群C222(1)，晶胞参数a = 123.2，b = 225.4，c = 72.4 Å。
