Alanko T, Rosenberg M, Saksela O
Department of Virology, Haartman Institute, University of Helsinki, Finland.
J Invest Dermatol. 1999 Jul;113(1):111-6. doi: 10.1046/j.1523-1747.1999.00636.x.
Melanocytes, the pigment forming cells of the skin, form an almost nonproliferating cell population located to the lowermost part of the epidermis. Normally melanocytes are not found higher in the epidermis or in the dermis. Nevi consist of melanocytes with altered growth characteristics and localization. The common pigmented nevus, a benign skin lesion, develops when melanocytes proliferate in the dermo-epidermal junction or in the dermis. Here we report growth characteristics of in vitro cultured normal human melanocytes and dermal nevus-derived melanocytes. As previously reported, nevus cells have a moderate to high FGF-2 expression level. Here we demonstrate that dermal nevus cells are able to survive in three-dimensional type 1 collagen culture, while normal human melanocytes rapidly undergo apoptosis. Melanocytes also, however, survive in collagen cultures in the presence of exogenous FGF-2. The survival of nevus cells in collagen is suppressed by protamine, an inhibitor of FGF-mediated cell stimulation. The in vivo growth environment of dermal nevus cells consists largely of type I and type III collagens. The results suggest that FGF-2 expression by nevus cells allows them to adapt to grow in the dermis. FGF-2 obviously has importance as a melanocyte survival factor and probably also in the development of malignant melanoma.
黑素细胞是皮肤中形成色素的细胞，构成了位于表皮最下层的一个几乎不增殖的细胞群体。正常情况下，在表皮较高部位或真皮中找不到黑素细胞。痣由生长特性和定位发生改变的黑素细胞组成。普通色素痣是一种良性皮肤病变，当黑素细胞在真皮 - 表皮交界处或真皮中增殖时就会形成。在此我们报告体外培养的正常人黑素细胞和真皮痣来源的黑素细胞的生长特性。如先前报道，痣细胞具有中等至高的FGF - 2表达水平。在此我们证明真皮痣细胞能够在三维I型胶原培养物中存活，而正常人黑素细胞会迅速发生凋亡。然而，在存在外源性FGF - 2的情况下，黑素细胞也能在胶原培养物中存活。鱼精蛋白（一种FGF介导的细胞刺激抑制剂）可抑制痣细胞在胶原中的存活。真皮痣细胞的体内生长环境主要由I型和III型胶原组成。结果表明，痣细胞表达的FGF - 2使它们能够适应在真皮中生长。FGF - 2显然作为黑素细胞存活因子具有重要意义，并且可能在恶性黑色素瘤的发生发展中也具有重要作用。
