Knott V J, Howson A L, Perugini M, Ravindran A V, Young S N
Department of Psychiatry, University of Ottawa, Ontario, Canada.
Biol Psychiatry. 1999 Jul 15;46(2):229-38. doi: 10.1016/s0006-3223(98)00338-2.
Efforts to model putative serotonergic deficits associated with affective disorders have frequently involved acute tryptophan depletion (ATD) as a manipulation strategy aimed at lowering brain serotonin synthesis. In an attempt to widen the scope of the measurement probes used in these investigations, the central actions of ATD and a subsequent dose of fenfluramine were examined via utilization of quantitative electroencephalography (EEG) and mood ratings.
Electroencephalograms (EEG) and subjective mood ratings were assessed in 28 healthy men before and after double-blind ingestion of a tryptophan-depleting (T-) amino acid mixture, or a nutritionally balanced (B) amino acid mixture containing tryptophan, and again after a single-blind oral dose of D,L-fenfluramine hydrochloride (60 mg).
Compared to the B mixture, the T- mixture reduced total plasma tryptophan by more than 75% 5 hours after ingestion. Tryptophan depletion was associated with a modest lowering of mood and a slowing of EEG as indicated by increases in delta amplitude. Fenfluramine caused no change in mood but increased fast wave (beta) activity in anterior recordings when administered after the T-, but not after the B mixture.
Quantitative EEG measurements may be a promising method for studying the central mechanisms underlying serotonin-mediated changes in mood and behavior.
模拟与情感障碍相关的假定血清素缺乏的研究工作，经常涉及急性色氨酸耗竭（ATD），作为一种旨在降低脑血清素合成的操作策略。为了拓宽这些研究中使用的测量探针的范围，通过利用定量脑电图（EEG）和情绪评分，研究了ATD以及随后一剂芬氟拉明的中枢作用。
在28名健康男性双盲摄入色氨酸耗竭（T-）氨基酸混合物或含色氨酸的营养均衡（B）氨基酸混合物之前和之后，以及单盲口服一剂盐酸D，L-芬氟拉明（60毫克）之后，评估脑电图（EEG）和主观情绪评分。
与B混合物相比，摄入后5小时，T-混合物使总血浆色氨酸降低了75%以上。色氨酸耗竭与情绪适度降低和脑电图减慢有关，表现为δ波幅增加。芬氟拉明在T-混合物之后给药时，对情绪无影响，但增加了前部记录中的快波（β）活动，而在B混合物之后给药则无此现象。
定量脑电图测量可能是研究血清素介导的情绪和行为变化潜在中枢机制的一种有前景的方法。
