Chulze S N, Etcheverry M G, Lecumberry S E, Magnoli C E, Dalcero A M, Ramirez M L, Pascale M, Rodriguez M I
Dpto. de Microbiología e Inmunología, Facultad de Ciencias Exactas Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
J Food Prot. 1999 Jul;62(7):814-7. doi: 10.4315/0362-028x-62.7.814.
Production of fumonisins B1, B2, and B3 by Fusarium moniliforme was evaluated on irradiated corn kernels inoculated with different spore concentrations (10, 10(2), 10(3), 10(5), and 10(6)), a water activity of 0.97, and a temperature of 25 degrees C. There was a direct relationship between the level of toxin produced and inoculum size. The highest levels of total fumonisin produced after 35 days of incubation were 5,028 and 9,063 ng/g at 10(5) and 10(6) spores per ml, respectively. The pattern of fumonisin production (FB1 > FB2 > FB3) in cultures growing from different inocula was not affected during the 35 days of incubation. The ratio between FB2 and FB1 varied from 0.15 to 0.42, whereas the ratio between FB3 and FB1 varied from 0.34 to 0.87.
在接种了不同孢子浓度(10、10²、10³、10⁵和10⁶)、水分活度为0.97且温度为25℃的辐照玉米粒上,评估了串珠镰刀菌产生伏马毒素B1、B2和B3的情况。毒素产生水平与接种量之间存在直接关系。培养35天后,每毫升10⁵和10⁶个孢子时,伏马毒素总产量的最高水平分别为5028和9063纳克/克。在35天的培养期内,不同接种量培养物中伏马毒素的产生模式(FB1 > FB2 > FB3)未受影响。FB2与FB1的比值在0.15至0.42之间变化,而FB3与FB1的比值在0.34至0.87之间变化。