Wang Y D, Chen H B, Jin M, Ou B, Kashiwagi K, Tsukahara S
Department of Ophthalmology, Yamanashi Medical University, Tamaho, Yamanashi, Japan.
Ophthalmic Res. 1999;31(5):378-86. doi: 10.1159/000055561.
The purpose of this study is to visualize the three-dimensional arrangement of collagen fibrils in aged human ciliary body and discuss their significance. The ciliary bodies obtained from two human eyes were treated with a NaOH cell-maceration method for 7 days, then prepared conventionally for light and scanning electron microscopy. The general morphology of the collagen tissue in the ciliary body appeared almost the same as that normally observed. Cellular elements were completely removed, but collagen fibrils were well preserved. In the stroma of the ciliary body, collagen fibrils were arranged irregularly. In the areas of the radial and circular ciliary muscles, considerable numbers of collagen fiber bundles were observed running in a circular direction. A honeycomb structure was seen in the pars plana, the walls and base of which were formed by interweaving collagen fibrils. The results suggested that collagen fibrils in the aged human ciliary body may be largely involved in the presbyopia.
本研究的目的是可视化老年人睫状体中胶原纤维的三维排列，并探讨其意义。从两只人眼中获取的睫状体采用氢氧化钠细胞浸渍法处理7天，然后按照常规方法制备用于光学显微镜和扫描电子显微镜观察。睫状体中胶原组织的总体形态与正常观察到的几乎相同。细胞成分被完全去除，但胶原纤维保存完好。在睫状体的基质中，胶原纤维排列不规则。在放射状和环状睫状肌区域，观察到相当数量的胶原纤维束呈环状排列。在睫状平坦部可见蜂窝状结构，其壁和底部由交织的胶原纤维形成。结果表明，老年人睫状体中的胶原纤维可能在老花眼中起很大作用。
