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[运用分类印记分析和随机引物聚合酶链反应对田鼠亚科(仓鼠科,啮齿目)田鼠进行遗传分化研究]

[Genetic differentiation of voles of the tribe Arvicolini (Cricetidae, Rodentia) using taxonprint analysis and polymerase chain reaction with random primers].

作者信息

Potapov S G, Orlov V N, Koval'skaia Iu M, Malygin V M, Ryskov A P

机构信息

Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russia.

出版信息

Genetika. 1999 Apr;35(4):484-92.

PMID:10420271
Abstract

The variability of the genomes of ten vole species was analyzed by means of taxonomic DNA fingerprinting and polymerase chain reaction using random primers (RAPD-PCR). The dendrograms of genetic similarity between the representatives of the tribe Arvicolinae (Gray, 1821) were constructed, based on the data obtained by means of both methods. The topology of the genetic similarity dendrogram that is based on the RAPD-PCR data generally correlates with the genetic distances estimated from biochemical and karyological data. The results did not confirm the genus status of Terricola. On the other hand, the data from taxonprint analysis suggest the recognition of Lasiopodomys as a separate genus.

摘要

利用分类DNA指纹图谱和随机引物聚合酶链反应(RAPD-PCR)分析了十种田鼠物种基因组的变异性。基于通过这两种方法获得的数据,构建了田鼠亚科(Gray,1821)代表物种之间的遗传相似性树状图。基于RAPD-PCR数据的遗传相似性树状图的拓扑结构通常与从生化和核型数据估计的遗传距离相关。结果未证实Terricola属的地位。另一方面,分类指纹分析的数据表明应将长尾仓鼠属(Lasiopodomys)识别为一个独立的属。

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