Endo T, Koizumi S, Tabata K, Kakita S, Ozaki A
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Japan.
Carbohydr Res. 1999 Mar 31;316(1-4):179-83. doi: 10.1016/s0008-6215(99)00050-6.
A large-scale production system of N-acetyllactosamine, a core structure of various oligosaccharides, was established by a whole-cell reaction through the combination of recombinant Escherichia coli strains and Corynebacterium ammoniagenes. Two recombinant E. coli strains over-expressed the UDP-Gal biosynthetic genes and the beta-(1-->4)-galactosyltransferase gene of Neisseria gonorrhoeae, respectively. C. ammoniagenes contributed the production of UTP from orotic acid. N-Acetyllactosamine was accumulated at 279 mM (107 g L-1) after a 38 h reaction (2.5 L in volume) starting from orotic acid, D-galactose, and 2-acetamido-2-deoxy-D-glucose.
通过重组大肠杆菌菌株和产氨棒杆菌的组合，利用全细胞反应建立了各种低聚糖核心结构N-乙酰乳糖胺的大规模生产系统。两种重组大肠杆菌菌株分别过表达淋病奈瑟菌的UDP-Gal生物合成基因和β-(1→4)-半乳糖基转移酶基因。产氨棒杆菌负责从乳清酸生产UTP。从乳清酸、D-半乳糖和2-乙酰氨基-2-脱氧-D-葡萄糖开始，经过38小时反应（体积2.5升）后，N-乙酰乳糖胺积累至279 mM（107克/升）。
