Cesareni G, Castagnoli L, Cestra G
Department of Biology, Enrico Calef, University of Rome Tor Vergata, Italy.
Comb Chem High Throughput Screen. 1999 Feb;2(1):1-17.
Peptide libraries may be constructed by grafting, in vitro, random DNA sequences into a carrier gene and then introducing the degenerate hybrid coding sequence into an expression organism. This review will focus on phage display, which was the first expression organism for peptide library expression to be described and which still maintains predominance in this area because of its simplicity, minimal cost, ease of manipulation, power and robustness. Using phage as the host, a repertoire of random peptides can be expressed that may be searched by a variety of screening or selection procedures. By physically associating each element of the peptide library with its coding sequence, selection for a property of a specific peptide results in the enrichment of the corresponding gene thus facilitating its cloning and amplification. This review focuses on the construction and screening of peptide libraries displayed on filamentous phage capsid and only briefly discusses the display of proteins and protein domains.
肽库可以通过在体外将随机DNA序列嫁接到载体基因中，然后将简并杂交编码序列导入表达生物体来构建。本综述将聚焦于噬菌体展示，它是首个被描述用于肽库表达的表达生物体，并且由于其简单性、低成本、易于操作、强大功能和稳健性，在该领域仍占据主导地位。以噬菌体作为宿主，可以表达一系列随机肽，这些肽可以通过各种筛选或选择程序进行筛选。通过将肽库的每个元件与其编码序列进行物理关联，对特定肽的性质进行选择会导致相应基因的富集，从而便于其克隆和扩增。本综述聚焦于丝状噬菌体衣壳上展示的肽库的构建和筛选，仅简要讨论蛋白质和蛋白质结构域的展示。
