Obón J M, Maiquez J R, Cánovas M, Kleber H P, Iborra J L
Department of Biochemistry and Molecular Biology B, Faculty of Chemistry, University of Murcia, Spain.
Appl Microbiol Biotechnol. 1999 Jun;51(6):760-4. doi: 10.1007/s002530051459.
The use of a biological procedure for L-carnitine production as an alternative to chemical methods must be accompanied by an efficient and highly productive reaction system. Continuous L-carnitine production from crotonobetaine was studied in a cell-recycle reactor with Escherichia coli O44 K74 as biocatalyst. This bioreactor, running under the optimum medium composition (25 mM fumarate, 5 g/l peptone), was able to reach a high cell density (26 g dry weight/l) and therefore to obtain high productivity values (6.2 g L-carnitine l-1 h-1). This process showed its feasibility for industrial L-carnitine production. In addition, resting cells maintained in continuous operation, with crotonobetaine as the only medium component, kept their biocatalytic capacity for 4 days, but the biotransformation capacity decreased progressively when this particular method of cultivation was used.
使用生物方法生产左旋肉碱作为化学方法的替代方案，必须配备高效且高产的反应系统。以大肠杆菌O44 K74作为生物催化剂，在细胞循环反应器中研究了从巴豆甜菜碱连续生产左旋肉碱的过程。该生物反应器在最佳培养基组成（25 mM富马酸盐，5 g/l蛋白胨）下运行，能够达到高细胞密度（26 g干重/l），因此获得了高生产率值（6.2 g L-1左旋肉碱 h-1）。该过程显示了其用于工业生产左旋肉碱的可行性。此外，以巴豆甜菜碱作为唯一培养基成分维持连续运行的静止细胞，其生物催化能力可保持4天，但采用这种特定培养方法时，生物转化能力会逐渐下降。
