Neufeld M V, Steinemann T L, Merin L M, Stroop W G, Brown M F
Department of Ophthalmology, University of Arkansas for Medical Sciences, Little Rock 72205-7199, USA.
Cornea. 1999 Jul;18(4):489-92. doi: 10.1097/00003226-199907000-00016.
To demonstrate the presence of an active herpetic dendrite in an eye-bank cornea.
One eye-bank cornea was studied. Viral cultures and polymerase chain reaction (PCR) testing were performed 4 days after tissue preservation. The presence or absence of herpes simplex virus (HSV) type 1 was investigated.
The presence of an active HSV dendrite in an eye-bank cornea was verified. HSV type 1 was confirmed using PCR amplification and restriction endonuclease DNA fragment analysis.
This case suggests that HSV may remain viable in stored corneal tissue at 4 degrees C.
证明眼库角膜中存在活动性疱疹性树枝状病变。
对一只眼库角膜进行了研究。组织保存4天后进行了病毒培养和聚合酶链反应（PCR）检测。调查了1型单纯疱疹病毒（HSV）的有无。
证实眼库角膜中存在活动性HSV树枝状病变。通过PCR扩增和限制性内切酶DNA片段分析确认了1型HSV。
该病例表明HSV在4℃保存的角膜组织中可能保持存活。
