Manfredi R, Borderi M, Re M C, Furlini G, Monari P, Spinosa Guzmán S M, Tadolini M, Talò S, Donzelli C, Maldini C, Chiodo F
Department of Clinical and Experimental Medicine, University of Bologna, Italy.
New Microbiol. 1999 Jul;22(3):269-75.
In order to compare HIV-1 p24 antigenemia and plasma HIV-1 RNA levels as markers of viral replication, 3,129 paired determinations of alkaline immunocomplex-dissociated serum HIV-1 p24 antigenemia (performed with an immunoenzymatic assay), and plasma HIV-1 RNA levels (carried out with a branched DNA method, a reverse transcriptase-coupled polymerase chain reaction, and a nucleic acid sequence-based assay) were assessed over a two-year-period. When excluding samples with undetectable plasma HIV-1 RNA levels (which tested negative or borderline positive at serum p24 antigen assay in 97.9% of cases), immunocomplex-dissociated p24 antigenemia proved significantly less sensitive than viral load at all considered HIV-1 RNA reference levels, although the profile of positive serum p24 antigen assays (values above 10 pg/ml) paralleled the trend of plasma HIV-1 viral load, especially at higher levels. However, serum HIV-1 p24 antigenemia (even after immunocomplex dissociation) can be longer suggested as a the sole virological tool in the laboratory management of HIV-1 infection, due to its significantly lower sensitivity levels compared with viral load assessment.
为了比较HIV-1 p24抗原血症和血浆HIV-1 RNA水平作为病毒复制标志物的情况，在两年期间对3129对样本进行了碱性免疫复合物解离血清HIV-1 p24抗原血症（采用免疫酶测定法）和血浆HIV-1 RNA水平（采用分支DNA法、逆转录酶偶联聚合酶链反应和基于核酸序列的测定法）的测定。当排除血浆HIV-1 RNA水平检测不到的样本（在血清p24抗原检测中97.9%的病例呈阴性或临界阳性）时，在所有考虑的HIV-1 RNA参考水平下，免疫复合物解离的p24抗原血症的敏感性均显著低于病毒载量，尽管血清p24抗原检测阳性（值高于10 pg/ml）的情况与血浆HIV--1病毒载量的趋势平行，尤其是在较高水平时。然而，血清HIV-1 p24抗原血症（即使在免疫复合物解离后）由于其与病毒载量评估相比敏感性显著较低，因此在HIV-1感染的实验室管理中，可能不再建议将其作为唯一的病毒学检测手段。
