Manukhina E B, Malyshev I Iu, Smirin B V, Mashina S Iu, Saltykova V A, Vanin A F
Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russia.
Izv Akad Nauk Ser Biol. 1999 Mar-Apr(2):211-5.
It is common knowledge that adaptation to hypobaric hypoxia provides for a number of protective NO-dependent effects in the organism. However, many aspects of its influence on NO metabolism remain unclear. In this work we studied the relationship between NO production and deposition in the course of adaptation to hypoxia. No production was assayed by the total concentration of nitrates and nitrites in rat serum, while the volume of NO depot was determined from the level of isolated aorta relaxation in response to diethyldithiocarbamate in vitro. In the course of adaptation to hypoxia, nitrate and nitrite concentration increases as well as NO deposition in the vascular wall. The volume of NO depot correlated reliably with the serum concentration of nitrates and nitrites. Hence, adaptation to hypoxia increases NO production and deposition, which can underlie its NO-dependent protective effects.
众所周知，机体对低压缺氧的适应会产生许多依赖一氧化氮（NO）的保护作用。然而，其对NO代谢影响的许多方面仍不清楚。在这项研究中，我们研究了缺氧适应过程中NO生成与沉积之间的关系。通过大鼠血清中硝酸盐和亚硝酸盐的总浓度来测定NO生成，而NO储备量则根据体外二乙基二硫代氨基甲酸盐作用下离体主动脉舒张水平来确定。在缺氧适应过程中，硝酸盐和亚硝酸盐浓度增加，血管壁中NO沉积也增加。NO储备量与血清中硝酸盐和亚硝酸盐浓度呈可靠的相关性。因此，缺氧适应会增加NO的生成和沉积，这可能是其依赖NO的保护作用的基础。
