Zsolnai A, Orbán L
Research Institute for Animal Breeding and Nutrition, Herceghalom, Hungary.
Electrophoresis. 1999 Jun;20(7):1462-8. doi: 10.1002/(SICI)1522-2683(19990601)20:7<1462::AID-ELPS1462>3.0.CO;2-0.
Complex DNA fragment patterns produced by random amplified polymorphic DNA (RAPD) assays were separated in agarose as well as polyacrylamide gels using a continuous buffer and two discontinuous buffer systems, with and without urea, respectively. The effect of very high field strength/current levels on the duration of separation, the relative mobilities of the selected bands, and the appearance of the pattern was tested. The use of discontinuous buffer systems resulted in sharper bands and better resolution at 30 min duration of separation for DNA fragments in the size range of 200-2000 base pairs. These observations could help researchers in the rapid separation of band patterns produced by polymerase chain reaction (PCR)-based methods utilized in small- to mid-scale genome searches.
通过随机扩增多态性DNA（RAPD）分析产生的复杂DNA片段模式，分别使用连续缓冲液和两种不连续缓冲液系统（分别含尿素和不含尿素），在琼脂糖凝胶和聚丙烯酰胺凝胶中进行分离。测试了极高场强/电流水平对分离持续时间、所选条带的相对迁移率以及图谱外观的影响。对于大小在200 - 2000碱基对范围内的DNA片段，使用不连续缓冲液系统在分离30分钟时可产生更清晰的条带和更好的分辨率。这些观察结果有助于研究人员在小规模到中等规模的基因组搜索中，快速分离基于聚合酶链反应（PCR）方法产生的条带模式。
