Horikawa I, Miwa T, Ishimaru T, Furukawa M, Kato T, Moriizumi T
Department of Otorhinolaryngology, School of Medicine, Kanazawa University, Takaramachi, Japan.
Neuroreport. 1999 Jul 13;10(10):2205-8. doi: 10.1097/00001756-199907130-00037.
The purpose of the present study was to examine immunohistochemically the expression of high-affinity nerve growth factor receptor (trkA) in the olfactory nervous system of developing mice and of colchicine-treated adult mice. Olfactory epithelia of embryos and neonates showed trkA immunoreactivity not only in basal cells but in receptor cells, with trkA-immunoreactive olfactory nerve fibers in the subepithelium and the bulb. In adults, trkA immunoreactivity was found only in basal cells of olfactory epithelia. Olfactory epithelia of colchicine-treated adult mice, however, exhibited appearance of trkA-immunoreactive receptor cells and increased trkA immunoreactivity in olfactory nerve fibers. These findings indicate that expression of trkA continually occurs in the olfactory nervous system during life and that trkA can be highly expressed during development.
本研究的目的是通过免疫组织化学方法检测发育中小鼠和秋水仙碱处理的成年小鼠嗅觉神经系统中高亲和力神经生长因子受体（trkA）的表达。胚胎和新生小鼠的嗅上皮不仅在基底细胞中显示trkA免疫反应性，在受体细胞中也有显示，并且在基底膜下和嗅球中有trkA免疫反应性的嗅神经纤维。在成年小鼠中，trkA免疫反应性仅在嗅上皮的基底细胞中发现。然而，秋水仙碱处理的成年小鼠的嗅上皮表现出trkA免疫反应性受体细胞的出现以及嗅神经纤维中trkA免疫反应性的增加。这些发现表明，trkA在整个生命过程中持续在嗅觉神经系统中表达，并且在发育过程中trkA可以高度表达。
