Higo N, Oishi T, Yamashita A, Matsuda K, Hayashi M
Neuroscience Section, Information Science Division, Electrotechnical Laboratory, Umezono, Tsukuba, Ibaraki, Japan.
Cereb Cortex. 1999 Jun;9(4):317-31. doi: 10.1093/cercor/9.4.317.
We performed non-radioactive in situ hybridization histochemistry in several areas that include both the association areas and the lower sensory areas of monkey cerebral cortex, and investigated the localization of neurons expressing two growth-associated proteins: GAP-43 and SCG10. Both GAP-43 and SCG10 mRNAs were observed in both pyramidal and non-pyramidal neurons. Prominent hybridization signals for GAP-43 mRNA were observed in layers II-VI of the adult association areas: the prefrontal areas (FD), the temporal (TE) and the parietal (PG) association areas. The signals for GAP-43 mRNA were weak in layers I-III of the adult primary somatosensory area (PB) and primary (OC) and secondary (OB) visual areas, and cells with prominent signals were observed in layers IV-VI. The prominent signals for SCG10 mRNA were observed in layers IV-VI of all areas examined. These results suggest that the expression pattern of GAP-43 mRNA, but not that of SCG10 mRNA, may be related to the functional difference between association and lower sensory areas of adult cortex. In the infant cortex (postnatal days 2, 8 and 31), the signals for both mRNAs were intense in layers II-VI of all areas. Therefore, layer-specific expressions of GAP-43 and SCG10 mRNAs are established after the first postnatal month.
我们在包括猴子大脑皮层联合区和低级感觉区在内的多个区域进行了非放射性原位杂交组织化学实验，研究了表达两种生长相关蛋白：生长相关蛋白43（GAP - 43）和10号交感神经节肽（SCG10）的神经元的定位。在锥体神经元和非锥体神经元中均观察到了GAP - 43和SCG10的信使核糖核酸（mRNA）。在成年联合区的II - VI层观察到了明显的GAP - 43 mRNA杂交信号：前额叶区（FD）、颞叶联合区（TE）和顶叶联合区（PG）。在成年初级躯体感觉区（PB）、初级视觉区（OC）和次级视觉区（OB）的I - III层，GAP - 43 mRNA的信号较弱，而在IV - VI层观察到了信号明显的细胞。在所有检测区域的IV - VI层均观察到了明显的SCG10 mRNA信号。这些结果表明，GAP - 43 mRNA的表达模式而非SCG10 mRNA的表达模式可能与成年皮层联合区和低级感觉区之间的功能差异有关。在婴儿皮层（出生后第2、8和31天），所有区域的II - VI层中两种mRNA的信号都很强。因此，GAP - 43和SCG10 mRNA的层特异性表达是在出生后的第一个月之后建立的。
