利用碘-125放射性探测法在溶液中检测DNA-CRP复合物中的DNA扭结。
Detecting the DNA kinks in a DNA-CRP complex in solution with iodine-125 radioprobing.
作者信息
Karamychev V N, Zhurkin V B, Garges S, Neumann R D, Panyutin I G
机构信息
Nuclear Medicine Department, National Institutes of Health, Bethesda, Maryland 20892-1180, USA.
出版信息
Nat Struct Biol. 1999 Aug;6(8):747-50. doi: 10.1038/11519.
Auger-electron-emitting radioisotopes such as 125I produce DNA strand breaks within nanometer range of the decay site. Here we analyze these breaks in order to study changes in DNA conformation upon binding with cyclic AMP receptor protein (CRP) in solution. The clear difference we found in break frequency in the CRP-DNA complex, as compared to the naked DNA duplex, correlates with the increased distances between the deoxyriboses and the radioiodine atom caused by the CRP-induced kink observed in the cocrystal. Thus, we demonstrate that 125I radioprobing can be used to study fine conformational changes of DNA within DNA-protein complexes.
诸如125I之类的俄歇电子发射放射性同位素会在衰变位点的纳米范围内产生DNA链断裂。在此，我们分析这些断裂，以便研究溶液中与环磷酸腺苷受体蛋白（CRP）结合时DNA构象的变化。与裸露的DNA双链体相比，我们在CRP-DNA复合物的断裂频率中发现的明显差异，与共晶体中观察到的CRP诱导的扭结所导致的脱氧核糖与放射性碘原子之间距离增加有关。因此，我们证明125I放射性探测可用于研究DNA-蛋白质复合物中DNA的精细构象变化。
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