来自酿酒酵母突变体的内切多聚半乳糖醛酸酶的纯化与特性分析

Purification and characterization of an endo-polygalacturonase from a mutant of Saccharomyces cerevisiae.

作者信息

Hirose N, Kishida M, Kawasaki H, Sakai T

机构信息

Department of Applied Biochemistry, Faculty of Agriculture, Osaka Prefecture University, Japan.

出版信息

Biosci Biotechnol Biochem. 1999 Jun;63(6):1100-3. doi: 10.1271/bbb.63.1100.

DOI:10.1271/bbb.63.1100

PMID:10427698

Abstract

An extracellular endo-polygalacturonase (PGase) produced by a mutant of Saccharomyces cerevisiae was isolated. The enzyme was regarded, immunologically, as a PGase belonging to the Kluyveromyces marxianus group. The enzyme had properties similar to the PGase from K. marxianus in heat and pH stability, and N-terminal amino acid sequence. However, the enzyme showed different properties in optimum pH and temperature, molecular weight, and reactivity in antiserum against PGase from K. marxianus, indicating that the enzyme has a different molecular structure from the PGase from K. marxianus.

摘要

分离出了由酿酒酵母突变体产生的一种细胞外内切多聚半乳糖醛酸酶（PGase）。从免疫学角度来看，该酶被认为是属于马克斯克鲁维酵母组的一种PGase。该酶在热稳定性、pH稳定性和N端氨基酸序列方面具有与来自马克斯克鲁维酵母的PGase相似的特性。然而，该酶在最适pH和温度、分子量以及针对来自马克斯克鲁维酵母的PGase抗血清中的反应性方面表现出不同的特性，这表明该酶与来自马克斯克鲁维酵母的PGase具有不同的分子结构。