Hissner F, Mattusch J, Heinig K
University of Leipzig, Department of Biology, Germany.
J Chromatogr A. 1999 Jul 2;848(1-2):503-13. doi: 10.1016/s0021-9673(99)00458-6.
The analysis of sulfur species in complex matrices, like environmentally related samples, requires selective and sensitive as well as robust determination methods. As many as possible different anions need to be quantified in a reasonable analysis time. Besides ion chromatography, capillary electrophoresis has proven to be a very efficient technique for the separation and determination of ionic compounds. With the advantages of less sample and solvent consumption compared to conventional LC, short separation time, inexpensive and robust capillaries, CE was used to separate the anions sulfate, sulfite, thiosulfate, thiocyanate and sulfide. Detection and injection modes and the composition of the separation buffers have been varied to find the most suitable conditions. Conductivity detection after electrokinetic sample injection and improvement of calibration linearity allowed the determination of sulfur containing anions with low limits of detection (8 to 50 micrograms/l). The developed CE method was applied to the analysis of water from an open-pit mining lake.
在复杂基质（如环境相关样品）中对硫形态进行分析，需要选择性高、灵敏度高且稳健的测定方法。在合理的分析时间内，需要对尽可能多的不同阴离子进行定量。除了离子色谱法，毛细管电泳已被证明是一种用于分离和测定离子化合物的非常有效的技术。与传统液相色谱相比，毛细管电泳具有样品和溶剂消耗少、分离时间短、毛细管价格低廉且稳健等优点，因此被用于分离硫酸根、亚硫酸根、硫代硫酸根、硫氰酸根和硫离子等阴离子。对检测和进样模式以及分离缓冲液的组成进行了改变，以找到最合适的条件。电动进样后进行电导检测并改善校准线性，使得能够测定检测限低（8至50微克/升）的含硫阴离子。所开发的毛细管电泳方法应用于露天采矿湖湖水的分析。
