Jezek P, Zácková M, Reháková Z, Růzicka M, Borecký J, Skobisová E, Brucknerová J, Garlid K D, Gimeno R E, Tartaglia L A
Department of Membrane Transport Biophysics, Institute of Physiology, Prague, Czech Republic.
FEBS Lett. 1999 Jul 16;455(1-2):79-82. doi: 10.1016/s0014-5793(99)00853-4.
Antibodies against Escherichia coli-expressed uncoupling protein-2 (UCP2) and uncoupling protein-3 (UCP3) were raised by operating the blotted proteins into the spleen of minipigs. The antisera reacted more intensively with the recombinant UCP2 and UCP3 than with uncoupling protein-1 (UCP1) isolated from brown adipose tissue. Moreover, anti-UCP2 and cross-reacting anti-UCP3 antibodies identified the presence of the UCP2/3 antigen in isolated mitochondria from rat heart, rat kidney, rat brain, rabbit epididymal white adipose tissue, hamster brown adipose tissue, and rabbit skeletal muscle. It has been concluded that UCP2 is expressed in these tissues (UCP3 in skeletal muscle); however their existence in mitochondria had not previously been demonstrated.
通过将印迹蛋白注入小型猪的脾脏，制备了抗大肠杆菌表达的解偶联蛋白2（UCP2）和解偶联蛋白3（UCP3）的抗体。与从棕色脂肪组织分离的解偶联蛋白1（UCP1）相比，抗血清与重组UCP2和UCP3的反应更强烈。此外，抗UCP2和交叉反应的抗UCP3抗体在从大鼠心脏、大鼠肾脏、大鼠大脑、兔附睾白色脂肪组织、仓鼠棕色脂肪组织和兔骨骼肌分离的线粒体中鉴定出UCP2/3抗原的存在。得出的结论是，UCP2在这些组织中表达（UCP3在骨骼肌中）；然而，它们在线粒体中的存在此前尚未得到证实。
