Nakamura T, Ruiz-Lozano P, Lindner V, Yabe D, Taniwaki M, Furukawa Y, Kobuke K, Tashiro K, Lu Z, Andon N L, Schaub R, Matsumori A, Sasayama S, Chien K R, Honjo T
Department of Medical Chemistry, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606, Japan.
J Biol Chem. 1999 Aug 6;274(32):22476-83. doi: 10.1074/jbc.274.32.22476.
We have identified and characterized mouse, rat, and human cDNAs that encode a novel secreted protein of 448 amino acids named DANCE (developmental arteries and neural crest epidermal growth factor (EGF)-like). DANCE contains six calcium-binding EGF-like domains, one of which includes an RGD motif. Overexpression studies of recombinant DANCE protein document that DANCE is a secreted 66-kDa protein. DANCE and recently described protein S1-5 comprise a new EGF-like protein family. The human DANCE gene was mapped at chromosome 14q32.1. DANCE mRNA is mainly expressed in heart, ovary, and colon in adult human tissues. Expression profile analysis by in situ hybridization revealed prominent DANCE expression in developing arteries. DANCE is also expressed in neural crest cell derivatives, endocardial cushion tissue, and several other mesenchymal tissues. In adult vessels, DANCE expression is largely diminished but is reinduced in balloon-injured vessels and atherosclerotic lesions, notably in intimal vascular smooth muscle cells and endothelial cells that lose their ability to proliferate in late stage of injury. DANCE protein was shown to promote adhesion of endothelial cells through interaction of integrins and the RGD motif of DANCE. DANCE is thus a novel vascular ligand for integrin receptors and may play a role in vascular development and remodeling.
我们已经鉴定并表征了小鼠、大鼠和人类的cDNA，它们编码一种名为DANCE（发育性动脉和神经嵴表皮生长因子（EGF）样蛋白）的448个氨基酸的新型分泌蛋白。DANCE包含六个钙结合EGF样结构域，其中一个包含RGD基序。重组DANCE蛋白的过表达研究表明，DANCE是一种分泌型66 kDa蛋白。DANCE和最近描述的蛋白S1-5构成了一个新的EGF样蛋白家族。人类DANCE基因定位于染色体14q32.1。DANCE mRNA在成人组织中的心脏、卵巢和结肠中主要表达。原位杂交的表达谱分析显示，DANCE在发育中的动脉中有显著表达。DANCE也在神经嵴细胞衍生物、心内膜垫组织和其他几种间充质组织中表达。在成人血管中，DANCE的表达大大降低，但在球囊损伤的血管和动脉粥样硬化病变中重新诱导表达，特别是在损伤后期失去增殖能力的内膜血管平滑肌细胞和内皮细胞中。DANCE蛋白通过整合素与DANCE的RGD基序相互作用促进内皮细胞的粘附。因此，DANCE是整合素受体的一种新型血管配体，可能在血管发育和重塑中发挥作用。
