地塞米松抑制大鼠肝癌细胞中纤溶酶原激活物的机制。
Mechanism of dexamethasone inhibition of plasminogen activator in rat hepatoma cells.
作者信息
Seifert S C, Gelehrter T D
出版信息
Proc Natl Acad Sci U S A. 1978 Dec;75(12):6130-3. doi: 10.1073/pnas.75.12.6130.
Abstract
Glucocorticoids rapidly and completely inhibit intracellular plasminogen activator activity in rat hepatoma cells, as assayed by the solubilization of 125I-labeled fibrin. Experiments in which extracts of dexamethasone-treated cells are mixed with extracts of control cells demonstrate an inhibitor of protease activity. Plasminogen activator is found primarily in the particulate fraction of cell lysates, whereas the inhibitor is localized in the soluble fraction. Variant cells have been isolated previously that are fully resistant to the dexamethoasone inhibition of plasminogen activator activity. These variants have no demonstrable inhibitor activity, whereas plasminogen activator in these cells is fully sensitive to inhibitor from wild-type cells. Thus, the basis for hormone resistance appears to be the failure of dexamethasone to induce an inhibitor.
摘要
通过对125I标记的纤维蛋白进行溶解测定发现，糖皮质激素能迅速且完全抑制大鼠肝癌细胞内纤溶酶原激活剂的活性。将地塞米松处理过的细胞提取物与对照细胞提取物混合进行实验，结果显示存在一种蛋白酶活性抑制剂。纤溶酶原激活剂主要存在于细胞裂解物的颗粒部分，而抑制剂则定位于可溶性部分。先前已分离出对糖皮质激素抑制纤溶酶原激活剂活性完全耐药的变异细胞。这些变异细胞没有可检测到的抑制剂活性，而这些细胞中的纤溶酶原激活剂对野生型细胞的抑制剂完全敏感。因此，激素耐药的基础似乎是地塞米松未能诱导出抑制剂。
相似文献
1
Mechanism of dexamethasone inhibition of plasminogen activator in rat hepatoma cells.地塞米松抑制大鼠肝癌细胞中纤溶酶原激活物的机制。
Proc Natl Acad Sci U S A. 1978 Dec;75(12):6130-3. doi: 10.1073/pnas.75.12.6130.
2
Isolation of rat hepatoma cell variants selectively resistant to dexamethasone inhibition of plasminogen activator.
J Cell Physiol. 1979 Jun;99(3):333-41. doi: 10.1002/jcp.1040990308.
3
Characterization of the dexamethasone-induced inhibitor of plasminogen activator in HTC hepatoma cells.地塞米松诱导的HTC肝癌细胞纤溶酶原激活物抑制剂的特性
J Biol Chem. 1986 Mar 25;261(9):4352-7.
4
Hormonal regulation of plasminogen activator in rat hepatoma cells.大鼠肝癌细胞中纤溶酶原激活物的激素调节
Mol Cell Biochem. 1983;53-54(1-2):11-21. doi: 10.1007/BF00225243.
5
Dexamethasone inhibition of tissue-type plasminogen activator (tPA) activity: paradoxical induction of both tPA antigen and plasminogen activator inhibitor.
Mol Endocrinol. 1987 Jan;1(1):97-101. doi: 10.1210/mend-1-1-97.
6
Paradoxical effects of glucocorticoids on regulation of plasminogen activator activity of rat hepatoma cells.糖皮质激素对大鼠肝癌细胞纤溶酶原激活物活性调节的矛盾效应。
Proc Natl Acad Sci U S A. 1982 Apr;79(7):2319-22. doi: 10.1073/pnas.79.7.2319.
7
Dexamethasone induction of an inhibitor of plasminogen activator in HTC hepatoma cells.地塞米松诱导HTC肝癌细胞中纤溶酶原激活物抑制剂的产生。
J Biol Chem. 1984 Jun 10;259(11):6847-51.
8
Dexamethasone-induced adhesion in hepatoma cells: the role of plasminogen activator.地塞米松诱导肝癌细胞黏附：纤溶酶原激活剂的作用
Nature. 1979 Jan 25;277(5694):312-3. doi: 10.1038/277312a0.
9
Cyclic nucleotide regulation of plasminogen activator and plasminogen activator-inhibitor messenger RNAs in rat hepatoma cells.大鼠肝癌细胞中纤溶酶原激活物及纤溶酶原激活物抑制剂信使核糖核酸的环核苷酸调节
Mol Endocrinol. 1990 Jan;4(1):171-8. doi: 10.1210/mend-4-1-171.
引用本文的文献
1
A factor released by monocytes in the presence of dexamethasone stimulates neutrophil locomotion.单核细胞在存在地塞米松的情况下释放的一种因子会刺激中性粒细胞的移动。
Br J Pharmacol. 1993 Jan;108(1):248-54. doi: 10.1111/j.1476-5381.1993.tb13470.x.
2
Control of cell proliferation in human glioma by glucocorticoids.糖皮质激素对人胶质瘤细胞增殖的控制
Br J Cancer. 1980 Jun;41(6):857-66. doi: 10.1038/bjc.1980.161.
3
Isolation and characterization of dexamethasone-resistant mutants from human lymphoid cell line CEM-C7.从人淋巴细胞系CEM-C7中分离并鉴定地塞米松抗性突变体。
Mol Cell Biol. 1981 Jun;1(6):512-21. doi: 10.1128/mcb.1.6.512-521.1981.
4
Increased activity of plasminogen activators during involution of the rat ventral prostate.大鼠腹侧前列腺 involution 过程中纤溶酶原激活剂活性增加。 （注：“involution”在医学语境中可能有“退化”“复旧”等意思，这里结合前列腺的情况，可能是指其在特定阶段的某种生理变化过程，但仅从给定文本无法准确判断其确切含义，所以直接保留英文未翻译）
Biochem J. 1984 Jul 1;221(1):171-8. doi: 10.1042/bj2210171.
5
Structure and expression of the C3 gene.补体C3基因的结构与表达
Springer Semin Immunopathol. 1983;6(2-3):119-47. doi: 10.1007/BF00205869.
6
Regulation of rat urinary and renal kallikrein and prekallikrein by corticosteroids.皮质类固醇对大鼠尿和肾激肽释放酶及前激肽释放酶的调节作用。
Proc Natl Acad Sci U S A. 1983 May;80(10):3059-63. doi: 10.1073/pnas.80.10.3059.
7
Hormonal regulation of plasminogen activator in rat hepatoma cells.大鼠肝癌细胞中纤溶酶原激活物的激素调节
Mol Cell Biochem. 1983;53-54(1-2):11-21. doi: 10.1007/BF00225243.
8
Cyclic nucleotides, possible intracellular mediators of macrophage activation and secretory processes.环核苷酸，可能是巨噬细胞激活和分泌过程的细胞内介质。
Agents Actions. 1980 Dec;10(6):556-61. doi: 10.1007/BF02024165.
9
Transformation phenotype of polyoma virus-transformed rat fibroblasts: plasminogen activator production is modulated by the growth state of the cells and regulated by the expression of an early viral gene function.多瘤病毒转化的大鼠成纤维细胞的转化表型：纤溶酶原激活物的产生受细胞生长状态的调节，并受病毒早期基因功能表达的调控。
J Virol. 1980 Aug;35(2):420-7. doi: 10.1128/JVI.35.2.420-427.1980.
10
Protease-activated "prodrugs" for cancer chemotherapy.用于癌症化疗的蛋白酶激活“前药”。
Proc Natl Acad Sci U S A. 1980 Apr;77(4):2224-8. doi: 10.1073/pnas.77.4.2224.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Plasminogen: purification from human plasma by affinity chromatography.纤溶酶原：通过亲和色谱法从人血浆中纯化。
Science. 1970 Dec 4;170(3962):1095-6. doi: 10.1126/science.170.3962.1095.
3
Inhibition by hydrocortison of plasminogen activator production in rat tongue organ cultures.氢化可的松对大鼠舌器官培养物中纤溶酶原激活物产生的抑制作用。
Lab Invest. 1974 Apr;30(4):427-33.
4
Induction of tyrosine alpha-ketoglutarate transaminase by steroid hormones in a newly established tissue culture cell line.类固醇激素在新建立的组织培养细胞系中诱导酪氨酸α-酮戊二酸转氨酶的产生。
Proc Natl Acad Sci U S A. 1966 Jul;56(1):296-303. doi: 10.1073/pnas.56.1.296.
5
Mechanism of action of glucocorticoids.糖皮质激素的作用机制。
Metabolism. 1974 Feb;23(2):159-202. doi: 10.1016/0026-0495(74)90113-9.
6
Association of a protease (plasminogen activator) with a specific membrane fraction isolated from transformed cells.一种蛋白酶（纤溶酶原激活剂）与从转化细胞中分离出的特定膜组分的关联。
J Cell Biol. 1976 Nov;71(2):472-86. doi: 10.1083/jcb.71.2.472.
7
Fibrinolytic activity of adult rat liver cells in primary culture and inhibition by glucocorticoids.原代培养成年大鼠肝细胞的纤溶活性及糖皮质激素对其的抑制作用。
Biochem Biophys Res Commun. 1976 Sep 20;72(2):462-71. doi: 10.1016/s0006-291x(76)80065-4.
8
9
Hormonal regulation of membrane phenotype.膜表型的激素调节
J Supramol Struct. 1977;6(3):325-31. doi: 10.1002/jss.400060305.
10
Secretion of plasminogen activator by human polymorphonuclear leukocytes. Modulation by glucocorticoids and other effectors.人多形核白细胞纤溶酶原激活物的分泌。糖皮质激素和其他效应物的调节作用。
J Exp Med. 1977 Dec 1;146(6):1693-706. doi: 10.1084/jem.146.6.1693.
Zotero 插件