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多瘤病毒转化的大鼠成纤维细胞的转化表型：纤溶酶原激活物的产生受细胞生长状态的调节，并受病毒早期基因功能表达的调控。

Transformation phenotype of polyoma virus-transformed rat fibroblasts: plasminogen activator production is modulated by the growth state of the cells and regulated by the expression of an early viral gene function.

作者信息

Perbal B

出版信息

J Virol. 1980 Aug;35(2):420-7. doi: 10.1128/JVI.35.2.420-427.1980.

DOI:10.1128/JVI.35.2.420-427.1980
PMID:6255182
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC288827/
Abstract

The expression of two transformation parameters, namely, ability to grow in agar and plasminogen activator production, was studied in several rat fibroblasts transformed by either wild-type or thermo-sensitive (tsa and ts25) polyoma viruses. The production of plasminogen activator was found to be dependent upon the growth state of the infected cells during a period of several days after infection. The analysis of the transformed phenotype of 25 tsa transformants and of 19 ts25 transformants independently isolated under various growth conditions led to the conclusion that there is no correlation between the regulation processes involved in plasminogen activator production and ability to grow without anchorage. The results obtained also suggested that the production of plasminogen activator is under the control of a functional large T antigen.

摘要

在几种由野生型或温度敏感型（tsa和ts25）多瘤病毒转化的大鼠成纤维细胞中，研究了两个转化参数的表达，即琼脂中生长能力和纤溶酶原激活物的产生。发现纤溶酶原激活物的产生取决于感染后几天内受感染细胞的生长状态。对在各种生长条件下独立分离的25个tsa转化体和19个ts25转化体的转化表型进行分析后得出结论，纤溶酶原激活物产生所涉及的调节过程与无锚定生长能力之间没有相关性。所获得的结果还表明，纤溶酶原激活物的产生受功能性大T抗原的控制。

相似文献

1
Transformation phenotype of polyoma virus-transformed rat fibroblasts: plasminogen activator production is modulated by the growth state of the cells and regulated by the expression of an early viral gene function.多瘤病毒转化的大鼠成纤维细胞的转化表型：纤溶酶原激活物的产生受细胞生长状态的调节，并受病毒早期基因功能表达的调控。
J Virol. 1980 Aug;35(2):420-7. doi: 10.1128/JVI.35.2.420-427.1980.
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本文引用的文献

1
ISOLATION OF TEMPERATURE-SENSITIVE MUTANTS OF POLYOMA VIRUS.多瘤病毒温度敏感突变体的分离
Virology. 1965 Apr;25:669-71. doi: 10.1016/0042-6822(65)90098-x.
2
AGAR SUSPENSION CULTURE FOR THE SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS.用于多瘤病毒转化细胞选择性测定的琼脂悬浮培养
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Transformation of rat fibroblast cells with early mutants of polyoma (tsa) and simian virus 40 (tsA30): occurrence of either A or N transformants depends on the multiplicity of infection.用多瘤病毒（tsa）和猿猴病毒40（tsA30）的早期突变体对大鼠成纤维细胞进行转化：A或N转化体的出现取决于感染复数。
J Virol. 1980 Feb;33(2):909-11. doi: 10.1128/JVI.33.2.909-911.1980.
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Distinct transformation phenotypes induced by polyoma virus and simian virus 40 in rat fibroblasts and their control by an early viral gene function.多瘤病毒和猿猴病毒40在大鼠成纤维细胞中诱导的不同转化表型及其受病毒早期基因功能的调控
J Virol. 1980 Feb;33(2):697-707. doi: 10.1128/JVI.33.2.697-707.1980.
6
Proteolytic enzymes initiating cell division and escape from contact inhibition of growth.启动细胞分裂并逃避生长接触抑制的蛋白水解酶。
Nature. 1970 Jul 11;227(5254):170-1. doi: 10.1038/227170a0.
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Tumor-associated fibrinolysis. Abstracted comments.肿瘤相关纤维蛋白溶解。摘要评论。
Fed Proc. 1973 Dec;32(12):2174-5.
8
Increased protease levels in transformed cells: a casein overlay assay for the detection of plasminogen activator production.转化细胞中蛋白酶水平升高：一种用于检测纤溶酶原激活物产生的酪蛋白覆盖分析方法。
Cell. 1974 Jun;2(2):95-102. doi: 10.1016/0092-8674(74)90097-x.
9
Lack of correlation between fibrinolysis and the transformed state of cultured mammalian cells.纤溶作用与培养的哺乳动物细胞转化状态之间缺乏相关性。
Biochem Biophys Res Commun. 1974 Nov 27;61(2):621-7. doi: 10.1016/0006-291x(74)91002-x.
10
Plasminogen activator production accompanies loss of anchorage regulation in transformation of primary rat embryo cells by simian virus 40.在猿猴病毒40转化原代大鼠胚胎细胞的过程中，纤溶酶原激活物的产生伴随着锚定调控的丧失。
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4792-6. doi: 10.1073/pnas.71.12.4792.