多瘤病毒转化的大鼠成纤维细胞的转化表型：纤溶酶原激活物的产生受细胞生长状态的调节，并受病毒早期基因功能表达的调控。
Transformation phenotype of polyoma virus-transformed rat fibroblasts: plasminogen activator production is modulated by the growth state of the cells and regulated by the expression of an early viral gene function.
作者信息
Perbal B
出版信息
J Virol. 1980 Aug;35(2):420-7. doi: 10.1128/JVI.35.2.420-427.1980.
Abstract
The expression of two transformation parameters, namely, ability to grow in agar and plasminogen activator production, was studied in several rat fibroblasts transformed by either wild-type or thermo-sensitive (tsa and ts25) polyoma viruses. The production of plasminogen activator was found to be dependent upon the growth state of the infected cells during a period of several days after infection. The analysis of the transformed phenotype of 25 tsa transformants and of 19 ts25 transformants independently isolated under various growth conditions led to the conclusion that there is no correlation between the regulation processes involved in plasminogen activator production and ability to grow without anchorage. The results obtained also suggested that the production of plasminogen activator is under the control of a functional large T antigen.
摘要
在几种由野生型或温度敏感型（tsa和ts25）多瘤病毒转化的大鼠成纤维细胞中，研究了两个转化参数的表达，即琼脂中生长能力和纤溶酶原激活物的产生。发现纤溶酶原激活物的产生取决于感染后几天内受感染细胞的生长状态。对在各种生长条件下独立分离的25个tsa转化体和19个ts25转化体的转化表型进行分析后得出结论，纤溶酶原激活物产生所涉及的调节过程与无锚定生长能力之间没有相关性。所获得的结果还表明，纤溶酶原激活物的产生受功能性大T抗原的控制。
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