van Rees B P, Musler A, Caspers E, Drillenburg P, Craanen M E, Polkowski W, Chibowski D, Offerhaus G J
Department of Pathology, Academic Medical Center, University of Amsterdam, The Netherlands.
Hepatogastroenterology. 1999 May-Jun;46(27):2063-8.
BACKGROUND/AIMS: Partial gastrectomy is a well-established pre-malignant condition. It is postulated that in the gastric stump an accelerated neoplastic process takes place, similar to that of (intestinal type) adenocarcinoma from the non-operated stomach. K-ras codon 12 mutation is one of the most frequent oncogenic alterations in human solid neoplasms. It is rare in conventional gastric carcinoma and has not been studied in gastric stump carcinoma. The aim of this study was to compare the prevalence of K-ras codon 12 point mutations in gastric stump carcinomas with those in conventional carcinomas from the non-operated stomach.
Twenty-four gastric stump carcinomas were compared with 26 conventional gastric carcinomas. Stage, histology, and demographics were comparable in both groups. Mutations in codon 12 of the K-ras gene were examined with a polymerase chain reaction (PCR)-based method and subsequent dot blot hybridization with mutation-specific probes. The results of Helicobacter pylori infection, Epstein-Barr virus infection and p53 immunohistochemistry were partially known from a previous study.
In one of the gastric stump carcinomas as well as in one of the conventional gastric carcinomas a K-ras codon 12 point mutation was found. p53 immunohistochemistry results were comparable in both groups. Interestingly, Helicobacter pylori infection rate and Epstein-Barr virus in situ hybridization for EBER1, as previously studied, appeared were significantly different in the two groups.
K-ras codon 12 point mutations are rare in both gastric stump carcinomas and conventional gastric carcinomas. This supports the postulated hypothesis that the pathways of carcinogenesis in both gastric stump carcinoma and conventional gastric carcinoma share common features. However, these groups differ in infection rate of Helicobacter pylori and of Epstein-Barr virus, which suggests that some neoplastic stimuli differ as well.
背景/目的：部分胃切除术是一种公认的癌前病变。据推测，在胃残端会发生加速的肿瘤形成过程，类似于未手术的胃（肠型）腺癌的过程。K-ras密码子12突变是人类实体瘤中最常见的致癌改变之一。它在传统胃癌中很少见，尚未在胃残端癌中进行研究。本研究的目的是比较胃残端癌与未手术胃的传统癌中K-ras密码子12点突变的发生率。
将24例胃残端癌与26例传统胃癌进行比较。两组的分期、组织学和人口统计学特征具有可比性。采用基于聚合酶链反应（PCR）的方法检测K-ras基因密码子12的突变，并随后用突变特异性探针进行斑点杂交。幽门螺杆菌感染、EB病毒感染和p53免疫组化的结果部分来自先前的一项研究。
在1例胃残端癌和1例传统胃癌中发现了K-ras密码子12点突变。两组的p53免疫组化结果具有可比性。有趣的是，如先前研究所示，两组的幽门螺杆菌感染率和EBER1的EB病毒原位杂交结果存在显著差异。
K-ras密码子12点突变在胃残端癌和传统胃癌中均很少见。这支持了这样一个假设，即胃残端癌和传统胃癌的致癌途径具有共同特征。然而，这些组在幽门螺杆菌和EB病毒的感染率方面存在差异，这表明一些致癌刺激也有所不同。
