Kispert A, Stöger R J, Caparros M, Herrmann B G
Max-Planck-Institut für Immunbiologie, Abt. Molekulare Embryologie, Stübeweg 51, 79108 Freiburg, Germany.
Mamm Genome. 1999 Aug;10(8):794-802. doi: 10.1007/s003359901094.
A variant form of mouse Chromosome (Chr) 17, the t-haplotype, contains several loci responsible for transmission ratio distortion in males. Sperm carrying the responder locus (Tcr) have a high probability of fertilizing eggs at the expense of wild-type sperm, provided that distorter loci (Tcd-1 to Tcd-5) are expressed during spermatogenesis. Tcr has been mapped to the Leh66b region within a maximum of 155 kb. In the search for genes in the genomic region Leh66EI, we have identified the mouse homolog of human ribosome S6 kinase 3 (RSK3) on cosmid DNA. The complete mouse Rsk3 gene is encoded in the region Leh66a of t-haplotypes and Leh66EI of the wild-type chromosome. It consists of at least 13 exons spanning over more than 120 kb. Rsk3 is expressed in embryos and in several adult organs including testis. Cosmids covering 100 kb of the Leh66b region or 120 kb of the Leh66a region were isolated. Rsk3 covers about 65 kb of the Leh66b region and appears to be incomplete at its 5'-end. A correlation of the physical map provided here with the genetic mapping of Tcr reported previously suggests that Tcr is most likely encoded within a fragment of 30 kb upstream or 20 kb downstream of Rsk3. These data will facilitate the isolation of Tcr, a prerequisite for understanding transmission ratio distortion in mouse.
小鼠17号染色体（Chr）的一种变异形式，即t单倍型，包含几个导致雄性传递率畸变的位点。携带应答位点（Tcr）的精子有很高的概率使卵子受精，而以野生型精子为代价，前提是畸变位点（Tcd - 1至Tcd - 5）在精子发生过程中表达。Tcr已被定位到Leh66b区域内，最大范围为155 kb。在搜索Leh66EI基因组区域中的基因时，我们在黏粒DNA上鉴定出了人类核糖体S6激酶3（RSK3）的小鼠同源物。完整的小鼠Rsk3基因编码在t单倍型的Leh66a区域和野生型染色体的Leh66EI区域。它至少由13个外显子组成，跨越超过120 kb。Rsk3在胚胎和包括睾丸在内的几个成年器官中表达。分离出了覆盖Leh66b区域100 kb或Leh66a区域120 kb的黏粒。Rsk3覆盖Leh66b区域约65 kb，并且在其5'端似乎不完整。此处提供的物理图谱与先前报道的Tcr遗传图谱的相关性表明，Tcr最有可能编码在Rsk3上游30 kb或下游20 kb的片段内。这些数据将有助于分离Tcr，这是理解小鼠传递率畸变的先决条件。
