Gustafson C J, Kratz G
Department of Reconstructive Plastic Surgery, Karolinska Hospital, Stockholm, Sweden.
Burns. 1999 Jun;25(4):331-5. doi: 10.1016/s0305-4179(99)00004-2.
The purpose of this study was to establish a method for transplantation of autologous keratinocytes on an allogenic cell-free dermis. From four healthy volunteers two full thickness skin grafts, 1 x 1 cm, were taken. The epidermis was separated from the dermis enzymatically and the cells of the dermal part were removed by incubation in Triton X-100. Keratinocytes were seeded on a cell-free dermis and the combined graft transplanted back to one of the wounds of the donor of the keratinocytes. The other wound was covered with cell-free dermis without keratinocytes. After 2, 3, 4 and 6 weeks, respectively, the grafted wounds were removed from the subjects and investigated histologically and immunohistochemically regarding re-epithelialisation, fibroblast ingrowth and angiogenesis. The wounds covered with cell-free dermis and keratinocytes showed a complete epidermal coverage 2 weeks after transplantation, in contrast to the wounds covered with un-seeded dermis which only showed epidermal coverage at the wound edges. There was also a marked difference concerning fibroblast ingrowth and angiogenesis. In this study we have shown that autologous keratinocytes can be seeded on a cell-free dermis and transplanted as a kerato-dermal graft which stimulate re-epithelialisation as well as fibroblast ingrowth and angiogenesis in the wound.
本研究的目的是建立一种将自体角质形成细胞移植到同种异体无细胞真皮上的方法。从四名健康志愿者身上取下两片1×1厘米的全层皮肤移植物。通过酶解法将表皮与真皮分离，并将真皮部分的细胞在Triton X-100中孵育以去除。将角质形成细胞接种到无细胞真皮上，然后将组合移植物移植回角质形成细胞供体的其中一个伤口。另一个伤口用无角质形成细胞的无细胞真皮覆盖。分别在2周、3周、4周和6周后，从受试者身上取下移植的伤口，进行组织学和免疫组织化学检查，观察再上皮化、成纤维细胞长入和血管生成情况。与仅在伤口边缘显示表皮覆盖的未接种真皮覆盖的伤口相比，无细胞真皮和角质形成细胞覆盖的伤口在移植后2周显示出完整的表皮覆盖。在成纤维细胞长入和血管生成方面也存在明显差异。在本研究中，我们表明自体角质形成细胞可以接种到无细胞真皮上，并作为角皮移植进行移植，从而刺激伤口的再上皮化以及成纤维细胞长入和血管生成。
