de Curtis M, Takashima I, Iijima T
Department of Experimental Neurophysiology, Istituto Nazionale Neurologico, via Celoria 11, 20133, Milan, Italy.
Brain Res. 1999 Aug 7;837(1-2):314-9. doi: 10.1016/s0006-8993(99)01712-6.
Cortical neuronal architecture and connectivity can be analyzed with high-resolution optical imaging after staining the in vitro isolated guinea pig brain preparation by circulating the voltage-sensitive dye RH795 via the arterial system. To establish this new technique, electrical field potentials evoked in the piriform and entorhinal cortices by lateral olfactory tract stimulation were correlated to the optical signal. The depth analysis of the optical response was performed by evaluating the contribution of the mono- and poly-synaptic components of the signal generated in different layers after applying a pair-pulse stimulation protocol. The tangential propagation of neuronal activity in olfactory cortices was evaluated by gathering several 4.2 x 4.2 mm images recorded from adjacent cortical areas. The real-time optical imaging technique applied to the isolated guinea pig brain can be successfully utilized to study the integrative properties of cortical neurons ensembles.
通过动脉系统循环电压敏感染料RH795，对体外分离的豚鼠脑标本进行染色后，可利用高分辨率光学成像分析皮质神经元结构和连接性。为建立这项新技术，将外侧嗅束刺激在梨状皮质和内嗅皮质诱发的电场电位与光学信号进行关联。通过评估双脉冲刺激方案应用后不同层产生的信号单突触和多突触成分的贡献，对光学响应进行深度分析。通过收集从相邻皮质区域记录的几个4.2×4.2毫米图像，评估嗅觉皮质中神经元活动的切线传播。应用于分离豚鼠脑的实时光学成像技术可成功用于研究皮质神经元群体的整合特性。
