Hanke T, Takizawa H, McMahon C W, Busch D H, Pamer E G, Miller J D, Altman J D, Liu Y, Cado D, Lemonnier F A, Bjorkman P J, Raulet D H
Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA.
Immunity. 1999 Jul;11(1):67-77. doi: 10.1016/s1074-7613(00)80082-5.
Mouse NK cells express at least seven inhibitory Ly49 receptors. Here we employ a semiquantitative cell-cell adhesion assay as well as class I/peptide tetramers to provide a comprehensive analysis of specificities of Ly49 receptors for class I MHC molecules in eight MHC haplotypes. Different Ly49 receptors exhibited diverse binding properties. The degree of class I binding was related to the extent of functional inhibition. The tetramer studies demonstrated that neither glycosylation nor coreceptors were necessary for class I binding to Ly49 receptors and uncovered peptide-specific recognition by a Ly49 receptor. The results provide a foundation for interpreting and integrating many existing functional studies as well as for designing tests of NK cell development and self-tolerance.
小鼠自然杀伤（NK）细胞表达至少七种抑制性Ly49受体。在此，我们采用半定量细胞间黏附试验以及I类/肽四聚体，对八种主要组织相容性复合体（MHC）单倍型中Ly49受体对I类MHC分子的特异性进行全面分析。不同的Ly49受体表现出不同的结合特性。I类结合程度与功能抑制程度相关。四聚体研究表明，糖基化和共受体对于I类与Ly49受体的结合并非必需，并揭示了Ly49受体对肽的特异性识别。这些结果为解释和整合许多现有的功能研究以及设计NK细胞发育和自身耐受性测试提供了基础。
