通过31P固态核磁共振研究马蜂毒素与磷脂膜的取向行为。
Orientational behavior of phospholipid membranes with mastoparan studied by 31P solid state NMR.
作者信息
Hori Y, Demura M, Niidome T, Aoyagi H, Asakura T
机构信息
Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Japan.
出版信息
FEBS Lett. 1999 Jul 23;455(3):228-32. doi: 10.1016/s0014-5793(99)00881-9.
Solid state 31P NMR spectroscopy was used to study the perturbing effect of the wasp venom peptide mastoparan (MP) on lipid bilayers composed of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylglycerol (DMPG). The 31P chemical shift anisotropy of multilamellar vesicles decreased with increasing peptide concentration, indicating that MP interacts strongly and selectively with the charged DMPG head group. Macroscopically oriented MP-lipid samples between glass plates were studied by 31P NMR as a function of tilt angle. These spectra showed the coexistence of orientation-dependent lamellar signals as well as an isotropic peak, suggesting that MP can induce non-lamellar phases in DMPC/DMPG membranes.
固态³¹P核磁共振光谱法被用于研究黄蜂毒液肽mastoparan（MP）对由二肉豆蔻酰磷脂酰胆碱（DMPC）和二肉豆蔻酰磷脂酰甘油（DMPG）组成的脂质双层的扰动作用。随着肽浓度的增加，多层囊泡的³¹P化学位移各向异性降低，这表明MP与带电荷的DMPG头部基团强烈且选择性地相互作用。通过³¹P核磁共振研究了玻璃板之间宏观取向的MP-脂质样品随倾斜角的变化。这些光谱显示了取向依赖性层状信号以及各向同性峰的共存，表明MP可在DMPC/DMPG膜中诱导非层状相。
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