Moran T M, Park H, Fernandez-Sesma A, Schulman J L
Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029, USA.
J Infect Dis. 1999 Sep;180(3):579-85. doi: 10.1086/314952.
Immunization with live influenza virus expands Th1 memory cells and facilitates more rapid recovery after heterosubtypic virus challenge. Immunization with inactivated virus generates a Th2 response and does not lead to heterosubtypic immunity. Creation of a Th1 priming environment by the inclusion of interleukin (IL)-12 with antibodies to IL-4 converted the response against inactivated virus to a Th1 response that was able to facilitate virus clearance upon heterosubtypic virus challenge. Evaluation of memory responses of mice immunized by the various protocols demonstrated that the type of immunization imprints T cell memory, dictating the nature of the response to subsequent infection. After live virus challenge, expansion of Th1 cells seems to facilitate the generation of cytotoxic T lymphocytes from naïve precursors. This latter finding may be the mechanism by which inactivated virus immunization in a Th1 cytokine context mediates heterosubtypic immunity.
用活流感病毒免疫可扩增Th1记忆细胞，并有助于在异型病毒攻击后更快恢复。用灭活病毒免疫会产生Th2反应，且不会导致异型免疫。通过将白细胞介素（IL）-12与抗IL-4抗体一起使用来创建Th1启动环境，可将针对灭活病毒的反应转变为Th1反应，这种反应能够在异型病毒攻击时促进病毒清除。对通过各种方案免疫的小鼠的记忆反应进行评估表明，免疫类型会印记T细胞记忆，决定对后续感染的反应性质。在活病毒攻击后，Th1细胞的扩增似乎有助于从幼稚前体产生细胞毒性T淋巴细胞。后一发现可能是在Th1细胞因子环境中进行灭活病毒免疫介导异型免疫的机制。
