Kalme T, Loukovaara M, Koistinen R, Koistinen H, Angervo M, Leinonen P, Seppälä M
Department of Obstetrics and Gynecology, Helsinki University Central Hospital, Finland.
Fertil Steril. 1999 Aug;72(2):325-9. doi: 10.1016/s0015-0282(99)00229-0.
To study the effects of E2 on insulin-like growth factor binding protein-1 (IGFBP-1) and sex hormone-binding globulin (SHBG) production with cultured human HepG2 hepatoma cells.
Experimental cell culture.
Academic medical center.
PATIENT(S): None.
INTERVENTION(S): Addition of E2 to cell culture medium.
MAIN OUTCOME MEASURE(S): Intracellular and released concentrations of IGFBP-1 and SHBG.
RESULT(S): Estradiol did not affect the intracellular or extracellular IGFBP-1 concentration, whereas the intracellular SHBG concentration increased significantly in response to 0.5-2.5 microM of E2.
CONCLUSION(S): Whereas the two binding proteins share a number of regulatory factors, their regulation by E2 is dissimilar in human hepatoma cells. Estradiol does not affect the intracellular or secreted IGFBP-1 concentration, but it does increase the production of SHBG.
