人类HLA-DR和-DQ分子在使用转基因小鼠的异种移植中的不同作用。
Different role of human HLA-DR and -DQ molecules in xenogeneic transplantation using transgenic mice.
作者信息
Sireci G, Dieli F, Di Sano C, Salerno A
机构信息
Istituto di Patologia Generale, University of Palermo, Italy.
出版信息
Transplantation. 1999 Jul 27;68(2):317-9. doi: 10.1097/00007890-199907270-00031.
BACKGROUND
The role of T lymphocytes in graft rejection in xenotransplantation is still unclear. The ability of the human HLA class II molecules DR and DQ to function as xenoantigens was investigated in a murine model of skin grafting, using HLA-DR1 and -DQ6-transgenic mice.
METHODS
Skin from HLA-DR1- or -DQ6-transgenic mice was transplanted in control littermates. Spleen cells from donors or recipients were tested in mixed lymphocyte reaction and cytotoxic assay.
RESULTS
Skin from HLA-DR1-transgenic mice was rejected and spleen cells from rejecting mice were able to proliferate to donor cells, although no rejection was observed when the skin of HLA-DQ6-transgenic mice was engrafted in control littermates. No cytotoxicity was observed in any models.
CONCLUSIONS
Taken all together these results clearly suggest a hierarchy in the xenogeneic potency of human HLA class II molecules, with the HLA-DR1 molecule functioning as a potent xenoantigen when compared with the HLA-DQ6 molecule.
背景
T淋巴细胞在异种移植排斥反应中的作用仍不清楚。使用HLA - DR1和 - DQ6转基因小鼠，在小鼠皮肤移植模型中研究了人类HLA II类分子DR和DQ作为异种抗原的功能。
方法
将HLA - DR1或 - DQ6转基因小鼠的皮肤移植到对照同窝小鼠体内。对供体或受体的脾细胞进行混合淋巴细胞反应和细胞毒性测定。
结果
HLA - DR1转基因小鼠的皮肤被排斥，排斥小鼠的脾细胞能够对供体细胞增殖，然而当将HLA - DQ6转基因小鼠的皮肤移植到对照同窝小鼠体内时未观察到排斥反应。在任何模型中均未观察到细胞毒性。
结论
综合所有这些结果清楚地表明人类HLA II类分子在异种抗原效力方面存在层级关系，与HLA - DQ6分子相比，HLA - DR1分子作为一种强效异种抗原发挥作用。
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