Artemov D, Pilatus U, Chu S, Mori N, Nelson J B, Bhujwalla Z M
Oncology Section, Division of MR Research, Department of Radiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Magn Reson Med. 1999 Aug;42(2):277-82. doi: 10.1002/(sici)1522-2594(199908)42:2<277::aid-mrm9>3.0.co;2-9.
Understanding the dynamics and pathogenesis of invasion is vital for developing strategies to prevent cancer metastasis. Conventional invasion assays provide information for a single time point. NMR microscopic imaging as used in the current study to measure cell invasion in vitro provides a nondestructive method for scoring cell invasion thus offering a unique possibility to study this process dynamically. An additional advantage is that cells can be retrieved for metabolic and physiological characterization. Two prostate cancer cell lines, DU-145 and Mat-Ly-Lu, preselected for differences in invasive behavior, were studied. Cells were seeded in 12-mm culture plate inserts containing a 15-microm-thick porous membrane with 3.0 microm pore size that was coated with a 100 microm Matrigel layer. Cell invasion in the Matrigel layer was obtained from the profile of intracellular water measured with diffusion-weighted 1D imaging. Additional experiments were also performed with confocal microscopy to validate the NMR results. Significant differences were detected between the invasive behavior of DU-145 and Mat-Ly-Lu cells. The obtained results show that NMR microscopy can be used to dynamically study invasion by cancer cells. The noninvasive nature of NMR microscopy permits determination of cell migration dynamically for any given sample, which is especially important if cell availability is limited to the unique sample, such as for biopsy specimens. Magn Reson Med 42:277-282, 1999.
了解侵袭的动态过程和发病机制对于制定预防癌症转移的策略至关重要。传统的侵袭检测方法只能提供单个时间点的信息。本研究中使用的核磁共振显微镜成像技术用于体外测量细胞侵袭，提供了一种无损的细胞侵袭评分方法，从而为动态研究这一过程提供了独特的可能性。另一个优点是可以获取细胞进行代谢和生理特征分析。研究了预先选择的具有不同侵袭行为的两种前列腺癌细胞系DU-145和Mat-Ly-Lu。将细胞接种在12毫米培养板插入物中，该插入物含有一个15微米厚、孔径为3.0微米的多孔膜，膜上涂有一层100微米的基质胶。通过扩散加权一维成像测量细胞内水的分布情况来获取基质胶层中的细胞侵袭情况。还进行了共聚焦显微镜的额外实验以验证核磁共振结果。检测到DU-145和Mat-Ly-Lu细胞的侵袭行为存在显著差异。所得结果表明，核磁共振显微镜可用于动态研究癌细胞的侵袭。核磁共振显微镜的非侵入性使得能够动态确定任何给定样品中的细胞迁移情况，这对于细胞可用性仅限于独特样品（如活检标本）的情况尤为重要。《磁共振医学》42:277 - 282, 1999年。
