神经母细胞瘤中14号染色体长臂上假定肿瘤抑制基因位点的亚定位

Sublocalization of putative tumor suppressor gene loci on chromosome arm 14q in neuroblastoma.

作者信息

Theobald M, Christiansen H, Schmidt A, Melekian B, Wolkewitz N, Christiansen N M, Brinkschmidt C, Berthold F, Lampert F

机构信息

Universitäts-Kinderklinik, Pädiatrische Hämatologie und Onkologie, Neuroblastom-Labor, Marburg, Germany.

出版信息

Genes Chromosomes Cancer. 1999 Sep;26(1):40-6.

PMID:10441004

Abstract

RFLP and microsatellite analysis with 23 polymorphic markers spanning the entire long arm of chromosome 14 in 108 neuroblastomas showed allelic loss in 19 out of 107 (18%) informative tumors, placing 14q among the most frequently affected chromosomal regions in neuroblastoma. One minimal deletion region could be sublocalized in 17 of 19 cases between markers D14S1 and D14S16, and a second one between markers D14S17 and D14S23 in band 14q32. Furthermore, breakpoints in bands 14q23 and 14q12 were detected. These results suggest the presence of at least two putative tumor suppressor gene loci on chromosome 14. Survival analyses revealed no prognostic impact of allelic loss of 14q in neuroblastoma. Genes Chromosomes Cancer 26:40-46, 1999.

摘要

对108例神经母细胞瘤进行RFLP和微卫星分析，使用23个跨越14号染色体整个长臂的多态性标记，结果显示，在107例具有信息性的肿瘤中有19例（18%）出现等位基因缺失，这使得14q成为神经母细胞瘤中最常受影响的染色体区域之一。19例中的17例，一个最小缺失区域可定位于标记D14S1和D14S16之间，另一个位于14q32带的标记D14S17和D14S23之间。此外，在14q23和14q12带检测到断点。这些结果表明14号染色体上至少存在两个假定的肿瘤抑制基因位点。生存分析显示，神经母细胞瘤中14q等位基因缺失对预后没有影响。《基因、染色体与癌症》26:40 - 46，1999年。