人类神经母细胞瘤中14号染色体长臂和1号染色体短臂的缺失图谱分析

Deletion mapping of chromosomes 14q and 1p in human neuroblastoma.

作者信息

Takayama H, Suzuki T, Mugishima H, Fujisawa T, Ookuni M, Schwab M, Gehring M, Nakamura Y, Sugimura T, Terada M

机构信息

National Cancer Center Research Institute, Tokyo, Japan.

出版信息

Oncogene. 1992 Jun;7(6):1185-9.

PMID:1594247

Abstract

It has been suggested that loss of heterozygosity (LOH) on the short arm of chromosome 1 is a critical event for the development of neuroblastoma, and we have previously shown frequent LOH on chromosome 14 in neuroblastoma. To pursue these observations, especially to define further the regions which are commonly deleted in the tumor, we examined for allelic losses in 27 cases of neuroblastomas by using a number of polymorphic DNA markers for chromosomes 14q and 1p. LOH was observed in 10 out of the 25 informative cases (40%) on chromosome 14q and in eight out of the 21 informative cases (38%) on 1p. The commonly deleted regions were distal to the D14S13 locus (14q32-qter) on chromosome 14 and distal to the D1S112 locus (1p36.1-pter) on chromosome 1. These results strongly suggest that tumor-suppressor genes important in the pathogenesis of human neuroblastoma are located on the distal part of both chromosomes 14q and 1p.

摘要

有人提出，1号染色体短臂上杂合性缺失（LOH）是神经母细胞瘤发生发展的关键事件，并且我们之前已表明神经母细胞瘤中14号染色体上存在频繁的LOH。为了进一步探讨这些发现，尤其是更精确地确定肿瘤中常见的缺失区域，我们使用了多个14q和1p染色体的多态性DNA标记，对27例神经母细胞瘤病例进行了等位基因缺失检测。在25例有信息价值的病例中，14q染色体上有10例（40%）观察到LOH，在21例有信息价值的病例中，1p染色体上有8例（38%）观察到LOH。常见的缺失区域位于14号染色体上D14S13位点（14q32 - qter）的远端以及1号染色体上D1S112位点（1p36.1 - pter）的远端。这些结果有力地表明，在人类神经母细胞瘤发病机制中起重要作用的肿瘤抑制基因位于14q和1p染色体的远端部分。