一种通过异源双链迁移分析（HMA）检测和区分临床重要酵母菌的快速方法。
A rapid method for detecting and distinguishing clinically important yeasts by heteroduplex mobility assays (HMAs).
作者信息
Olicio R, Almeida C A, Seuánez H N
机构信息
Genetics Section, Instituto Nacional de Câncer, Rio de Janeiro, Brazil.
出版信息
Mol Cell Probes. 1999 Aug;13(4):251-5. doi: 10.1006/mcpr.1999.0245.
Heteroduplex Mobility Assays (HMAs) of 700 bp amplified products of a 17S rDNA region were used to identify and differentiate seven yeast species of clinical importance Candida albicans, Torulopsis (Candida) glabrata, Candida tropicalis, Candida parapsilosis, Candida (Pichia) guilliermondii and Hansenula (Pichia) anomala. Distance of heteroduplex migration (dHE) was found to be negatively correlated to the number of nucleotide differences between amplified DNA sequences.
利用17S rDNA区域700 bp扩增产物的异源双链迁移分析（HMA）来鉴定和区分七种具有临床重要性的酵母菌种，即白色念珠菌、光滑假丝酵母（念珠菌属）、热带念珠菌、近平滑念珠菌、季也蒙念珠菌（毕赤酵母属）和异常汉逊酵母（毕赤酵母属）。发现异源双链迁移距离（dHE）与扩增DNA序列之间的核苷酸差异数量呈负相关。
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