通过聚合酶链反应（PCR）和核糖体DNA基因间隔区的限制性片段长度多态性分析鉴定念珠菌属菌种。
Identification of Candida species by PCR and restriction fragment length polymorphism analysis of intergenic spacer regions of ribosomal DNA.
作者信息
Williams D W, Wilson M J, Lewis M A, Potts A J
机构信息
Department of Oral Surgery, Medicine, and Pathology, Dental School, University of Wales College of Medicine, Cardiff, United Kingdom.
出版信息
J Clin Microbiol. 1995 Sep;33(9):2476-9. doi: 10.1128/jcm.33.9.2476-2479.1995.
Abstract
The PCR was used to amplify a targeted region of the ribosomal DNA from 84 Candida isolates. Unique product sizes were obtained for Candida guilliermondii, Candida (Torulopsis) glabrata, and Candida pseudotropicalis. Isolates of Candida albicans, Candida tropicalis, Candida stellatoidea, Candida parapsilosis, and Candida krusei could be identified following restriction digestion of the PCR products.
摘要
采用聚合酶链反应（PCR）从84株念珠菌分离株中扩增核糖体DNA的靶向区域。季也蒙念珠菌、光滑念珠菌（Torulopsis属）和伪热带念珠菌获得了独特的产物大小。白色念珠菌、热带念珠菌、星状念珠菌、近平滑念珠菌和克柔念珠菌的分离株可通过PCR产物的限制性酶切鉴定。
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