原核生物基因组大小与小亚基核糖体DNA拷贝数：从混合群体中估算微生物相对丰度
Prokaryotic Genome Size and SSU rDNA Copy Number: Estimation of Microbial Relative Abundance from a Mixed Population.
作者信息
Fogel GB, Collins CR, Li J, Brunk CF
机构信息
Natural Selection, Inc., 3333 North Torrey Pines Court, Suite 200, La Jolla, CA 92037, USA
出版信息
Microb Ecol. 1999 Aug;38(2):93-113. doi: 10.1007/s002489900162.
Abstract Determination of the relative abundance of a specific prokaryote in an environmental sample is of major interest in applied and environmental microbiology. Relative abundance can be calculated using knowledge of SSU rDNA copy number, amount of SSU rDNA in the sample, and a weighted average estimate of the genome sizes for organisms in the original sample. By surveying the literature, we provide estimates of genome size and SSU rDNA copy number for 303 and 101 prokaryotes, respectively. This compilation can be used to make reasonable estimates for a wide range of organisms in the calculation of relative abundance. A statistical analysis suggests that no correlation exists between genome size and SSU rDNA copy number. A phylogenetic analysis is used to offer insights into the evolution of both genome size and SSU rDNA copy number.http://link.springer-ny.com/link/service/journals/00248/bibs/38n2p93.html
摘要 在应用微生物学和环境微生物学中，测定环境样品中特定原核生物的相对丰度是一个重要的研究方向。相对丰度可以通过小亚基核糖体DNA（SSU rDNA）拷贝数、样品中SSU rDNA的量以及原始样品中生物基因组大小的加权平均估计值来计算。通过查阅文献，我们分别给出了303种原核生物的基因组大小估计值和101种原核生物的SSU rDNA拷贝数估计值。该汇编可用于在计算相对丰度时对多种生物进行合理估计。统计分析表明，基因组大小与SSU rDNA拷贝数之间不存在相关性。系统发育分析用于深入了解基因组大小和SSU rDNA拷贝数的进化。http://link.springer-ny.com/link/service/journals/00248/bibs/38n2p93.html
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