László F, Morschl E, Pávó I, Whittle B J
Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest.
Eur J Pharmacol. 1999 Jun 30;375(1-3):211-5. doi: 10.1016/s0014-2999(99)00233-2.
The actions of nitric oxide (NO) on gastrointestinal plasma loss, assessed by the leakage of [125I]human serum albumin, provoked by intraabdominal surgery and organ manipulation has been investigated in pentobarbitone-anaesthesized rats. Gentle manipulation (3 min) of the stomach or the small intestine following laparotomy leads to an increase in albumin extravasation in the stomach, duodenum, jejunum and colon over 1 h. Administration of the NO synthase inhibitors, N(G)-nitro-L-arginine methyl ester (1-5 mg kg(-1), s.c.) and N(G)-monomethyl-L-arginine (12.5-50 mg kg(-1), s.c.), provoked a further substantial elevation of gastrointestinal albumin extravasation in the surgically manipulated rat, but not in control rats. This effect could be prevented by the pretreatment (15 min) with L-arginine (300 mg kg(-1), s.c.) or by the concurrent infusion of the NO donor, S-nitroso-glutathione (5 microg kg(-1) min(-1), i.v.). Endogenous NO, most likely formed by endothelial NO synthase, thus appears to maintain microvascular integrity during surgery and organ manipulation of the gastrointestinal tract.
在戊巴比妥麻醉的大鼠中，研究了一氧化氮（NO）对因腹部手术和器官操作引起的胃肠道血浆丢失的作用，该作用通过[125I]人血清白蛋白的渗漏来评估。剖腹术后轻柔地操作（3分钟）胃或小肠会导致胃、十二指肠、空肠和结肠在1小时内白蛋白外渗增加。给予一氧化氮合酶抑制剂N（G）-硝基-L-精氨酸甲酯（1 - 5毫克/千克，皮下注射）和N（G）-单甲基-L-精氨酸（12.5 - 50毫克/千克，皮下注射），会使手术操作的大鼠胃肠道白蛋白外渗进一步大幅升高，但对对照大鼠无此作用。这种效应可通过预先（15分钟）给予L-精氨酸（300毫克/千克，皮下注射）或同时输注一氧化氮供体S-亚硝基谷胱甘肽（5微克/千克·分钟，静脉注射）来预防。因此，内源性一氧化氮很可能由内皮型一氧化氮合酶产生，在胃肠道手术和器官操作期间似乎能维持微血管完整性。
