Jin Y, Wang Y, Walker D L, Dong H, Conley C, Johansen J, Johansen K M
Department of Zoology and Genetics, Iowa State University, Ames 50011, USA.
Mol Cell. 1999 Jul;4(1):129-35. doi: 10.1016/s1097-2765(00)80195-1.
We have cloned and characterized JIL-1, a novel tandem kinase in Drosophila that associates with the chromosomes throughout the cell cycle. Antibody staining and live imaging of JIL-1-GFP transgenic flies show that JIL-1 localizes to the gene-rich interband regions of larval polytene chromosomes and is upregulated almost 2-fold on the hypertranscribed male X chromosome compared to autosomes. Phylogenetic analysis suggests that JIL-1 together with human MSKs defines a separate family of tandem kinases. That JIL-1 is a functional kinase was demonstrated by autophosphorylation and phosphorylation of histone H3 in vitro. Based on these findings, we propose that JIL-1 may play a role in transcriptional control potentially by regulating chromatin structure.
我们克隆并鉴定了JIL-1，它是果蝇中的一种新型串联激酶，在整个细胞周期中都与染色体相关联。对JIL-1-GFP转基因果蝇进行抗体染色和实时成像显示，JIL-1定位于幼虫多线染色体富含基因的间带区域，与常染色体相比，在转录活跃的雄性X染色体上其表达上调了近2倍。系统发育分析表明，JIL-1与人类MSKs共同定义了一个单独的串联激酶家族。JIL-1是一种功能性激酶，这在体外通过自身磷酸化和组蛋白H3的磷酸化得到了证实。基于这些发现，我们提出JIL-1可能通过调节染色质结构在转录控制中发挥作用。
