Resh M D
Cell Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, Box 143, New York, NY 10021, USA.
Biochim Biophys Acta. 1999 Aug 12;1451(1):1-16. doi: 10.1016/s0167-4889(99)00075-0.
Covalent attachment of myristate and/or palmitate occurs on a wide variety of viral and cellular proteins. This review will highlight the latest advances in our understanding of the enzymology of N-myristoylation and palmitoylation as well as the functional consequences of fatty acylation of key signaling proteins. The role of myristate and palmitate in promoting membrane binding as well as specific membrane targeting will be reviewed, with emphasis on the Src family of tyrosine protein kinases and alpha subunits of heterotrimeric G proteins. The use of myristoyl switches and regulated depalmitoylation as mechanisms for achieving reversible membrane binding and regulated signaling will also be explored.
肉豆蔻酸和/或棕榈酸的共价连接发生在多种病毒蛋白和细胞蛋白上。本综述将重点介绍我们对N-肉豆蔻酰化和棕榈酰化酶学的最新认识，以及关键信号蛋白脂肪酰化的功能后果。将综述肉豆蔻酸和棕榈酸在促进膜结合以及特定膜靶向中的作用，重点关注酪氨酸蛋白激酶的Src家族和异源三聚体G蛋白的α亚基。还将探讨使用肉豆蔻酰开关和调节性去棕榈酰化作为实现可逆膜结合和调节信号传导的机制。
