Ganesh Bhat B, Wang P, Kim J H, Black T M, Lewin T M, Fiedorek F T, Coleman R A
Departments of Nutrition, Pediatrics, and Medicine, The University of North Carolina, Chapel Hill NC 27599-7400, USA.
Biochim Biophys Acta. 1999 Aug 18;1439(3):415-23. doi: 10.1016/s1388-1981(99)00103-1.
Rat mitochondrial glycerol-3-phosphate acyltransferase (GPAT) cDNA was cloned and characterized. We identified a cDNA containing an open reading frame of 828 amino acids that had an 89% homology with the coding region of the previously characterized mouse mitochondrial GPAT and a predicted amino acid sequence that was 96% identical. The rat 5' UTR was only 159 nucleotides, in contrast to the 926 nucleotide 5' UTR of the mouse cDNA and had an internal deletion of 167 nucleotides. GPAT was expressed in Sf21 insect cells, and specific inhibitors strongly suggest that, like the Escherichia coli GPAT, the recombinant mitochondrial GPAT and the mitochondrial GPAT isoform in rat liver contain critical serine, histidine, and arginine residues.
大鼠线粒体甘油-3-磷酸酰基转移酶（GPAT）的cDNA被克隆并进行了表征。我们鉴定出一个包含828个氨基酸开放阅读框的cDNA，它与先前表征的小鼠线粒体GPAT的编码区具有89%的同源性，预测的氨基酸序列有96%的同一性。与小鼠cDNA的926个核苷酸的5'非翻译区（UTR）相比，大鼠的5'UTR只有159个核苷酸，并且有167个核苷酸的内部缺失。GPAT在Sf21昆虫细胞中表达，特异性抑制剂有力地表明，与大肠杆菌GPAT一样，重组线粒体GPAT和大鼠肝脏中的线粒体GPAT同工型含有关键的丝氨酸、组氨酸和精氨酸残基。
