Tokumura A, Fujimoto H, Yoshimoto O, Nishioka Y, Miyake M, Fukuzawa K
Faculty of Pharmaceutical Sciences, The University of Tokushima, Japan.
Life Sci. 1999;65(3):245-53. doi: 10.1016/s0024-3205(99)00243-x.
Lysophosphatidic acid has been identified as a vasopressor principle in incubated mammalian plasma and sera, and shown to be generated extracellulary by lysophospholipase D-like activity. In this study, we monitored the time course of changes in the major phospholipid fractions during incubation of plasma, and found that polyunsaturated lysophosphatidic acids accumulate more rapidly than saturated lysophosphatidic acids at expense of the corresponding lysophosphatidylcholines. We compared the phospholipase activities for producing bioactive LPA in age-matched spontaneously hypertensive rats and Wistar Kyoto rats. The lysophospholipase D activity in rat plasma was found to be independent of strain and age. We suggest that lysophospholipase D functions in rat for persistent production of bioactive LPA in the circulation throughout life. However, our finding that production of LPA in spontaneously hypertensive rats was not greater than that in Wistar Kyoto rats does not seem to support the idea that increased production of LPA is involved in the pathogenesis of hypertension.
溶血磷脂酸已被确认为孵育的哺乳动物血浆和血清中的一种血管加压物质，并显示通过类似溶血磷脂酶D的活性在细胞外生成。在本研究中，我们监测了血浆孵育过程中主要磷脂组分的变化时间进程，发现多不饱和溶血磷脂酸比饱和溶血磷脂酸积累得更快，代价是相应的溶血磷脂酰胆碱。我们比较了年龄匹配的自发性高血压大鼠和Wistar Kyoto大鼠中产生生物活性溶血磷脂酸的磷脂酶活性。发现大鼠血浆中的溶血磷脂酶D活性与品系和年龄无关。我们认为溶血磷脂酶D在大鼠中发挥作用，在整个生命过程中持续在循环中产生生物活性溶血磷脂酸。然而，我们发现自发性高血压大鼠中溶血磷脂酸的产生并不比Wistar Kyoto大鼠中的多，这似乎不支持溶血磷脂酸产生增加参与高血压发病机制的观点。
