Bachhawat N, Mande S C
Institute of Microbial Technology, Sector 39A, Chandigarh, 160036, India. nandita2lion.imtech.ernet.in
J Mol Biol. 1999 Aug 20;291(3):531-6. doi: 10.1006/jmbi.1999.2980.
1L-myo-inositol (inositol) is vital for the biogenesis of mycothiol, phosphatidylinositol and glycosylphosphatidylinositol anchors linked to complex carbohydrates in Mycobacterium tuberculosis. All these cellular components are thought to play important roles in host-pathogen interactions and in the survival of the pathogen within the host. However, the inositol biosynthetic pathway in M. tuberculosis is not known. To delineate the pathways for inositol formation, we employed a unique combination of tertiary structure prediction and yeast-based functional assays. Here, we describe the identification of the gene for mycobacterial INO1 that encodes inositol-1-phosphate synthase distinct in many respects from the eukaryotic analogues.
1L-肌醇对结核分枝杆菌中与复合碳水化合物相连的巯基肌醇、磷脂酰肌醇和糖基磷脂酰肌醇锚的生物合成至关重要。所有这些细胞成分都被认为在宿主-病原体相互作用以及病原体在宿主体内的存活中发挥重要作用。然而，结核分枝杆菌中的肌醇生物合成途径尚不清楚。为了描绘肌醇形成的途径，我们采用了三级结构预测和基于酵母的功能测定的独特组合。在这里，我们描述了分枝杆菌INO1基因的鉴定，该基因编码的肌醇-1-磷酸合酶在许多方面与真核类似物不同。
