Dodd S, Buist A, Burrows G D, Maguire K P, Norman T R
Department of Psychiatry, University of Melbourne, Austin and Repatriation Medical Centre, Victoria, Australia.
J Chromatogr B Biomed Sci Appl. 1999 Jul 9;730(2):249-55. doi: 10.1016/s0378-4347(99)00225-x.
A method is described for the determination of nefazodone and its active metabolites hydroxynefazodone, the dione BMS-180492 and m-chlorophenylpiperazine in blood plasma and expressed human milk based on reversed-phase high-performance liquid chromatography. Measurements were performed on drug-free plasma and expressed human milk spiked with nefazodone and metabolites to prepare and validate standard curves and specimens collected from nursing mothers. Parent drug and metabolites were separated from the biological matrices by solid-phase extraction using CERTIFY columns. Chromatographic separation was achieved with a C18 column and compounds were detected by their absorbance at 205 nm. Trazodone was used as an internal standard. The assay was validated for each analyte in the concentration range 200 to 1200 ng/ml.
描述了一种基于反相高效液相色谱法测定血浆和挤出人乳中奈法唑酮及其活性代谢物羟基奈法唑酮、二酮BMS-180492和间氯苯哌嗪的方法。对不含药物的血浆和添加了奈法唑酮及其代谢物的挤出人乳进行检测，以制备和验证标准曲线，并对哺乳期母亲采集的样本进行检测。母体药物及其代谢物通过使用CERTIFY柱的固相萃取从生物基质中分离出来。采用C18柱进行色谱分离，通过在205nm处的吸光度检测化合物。曲唑酮用作内标。该测定法在200至1200ng/ml的浓度范围内对每种分析物进行了验证。
