Thomas M G, Sylvester P A, Newcomb P, Longman R J
Academic Department of Surgery, Bristol Royal Infirmary, Bristol, UK.
J Clin Pathol. 1999 Mar;52(3):181-3. doi: 10.1136/jcp.52.3.181.
To determine whether the vitamin D receptor is expressed in colorectal cancer, and its relation to stage of disease.
Paraffin embedded sections of colorectal cancer from 30 patients who had undergone surgery were studied. Immunohistochemistry using the specific monoclonal antibody 9A7 gamma directed against the nuclear vitamin D receptor was used to identify receptors for the active metabolite of vitamin D3 (1,25-dihydroxyvitamin D3).
Microscopically normal human colorectal epithelium showed vitamin D receptor expression predominantly in the mid and upper crypts. All the colorectal cancer tissue studied showed vitamin D receptor expression, with a median of 25.3 (range 10.1 to 43.7) cells/graticule field (x 400). Although vitamin D receptor staining was heterogeneous within the individual cancers, neither Dukes stage nor the degree of differentiation appeared to influence expression of the receptor.
Colorectal cancer tissue expresses the nuclear vitamin D receptor and this could act as a potential therapeutic target for synthetic vitamin D3 differentiating agents.
确定维生素D受体是否在结直肠癌中表达及其与疾病分期的关系。
对30例接受手术的患者的结直肠癌石蜡包埋切片进行研究。使用针对核维生素D受体的特异性单克隆抗体9A7γ进行免疫组织化学，以鉴定维生素D3活性代谢物（1,25 - 二羟基维生素D3）的受体。
显微镜下正常的人类结肠直肠上皮显示维生素D受体主要在上皮隐窝的中上部表达。所有研究的结直肠癌组织均显示维生素D受体表达，每视野（×400）的细胞中位数为25.3（范围10.1至43.7）。尽管在个体癌症中维生素D受体染色不均匀，但无论是Dukes分期还是分化程度似乎都不影响受体的表达。
结直肠癌组织表达核维生素D受体，这可能成为合成维生素D3分化剂的潜在治疗靶点。
